摘要
为了探讨利用SCoT引物鉴别甜菜品种的可能性,利用3个多态性较好的SCoT引物对15个已经登记的甜菜品种进行扩增。结果表明3条引物SCoT2、SCoT12和SCoT21均表现出了很好的多态性,利用特征谱带法,SCoT12和SCoT21这两条引物单独使用均可鉴别全部的15个甜菜品种,而SCoT2也可以鉴别除了2号和9号以外的其它13个品种。利用SCoT分子标记技术可以实现较少引物快速鉴定甜菜品种真实性的目的,为保护甜菜育种家以及农民的利益,促进我国甜菜产业的健康发展发挥重要的作用。
In the paper,three selected SCoT primers were used to amplify DNA extracted from fifteen registered sugar beet varieties in order to explore the possibility of different variety identification.The results indicated that the polymorphic fragments could be amplified by SCoT2,SCoT12 and SCoT21.Both SCoT12 and SCoT21 could be used to identify all 15 registered varieties.The SCoT2 could identify 13 registered varieties except two varieties(No.2 and No.9).These results showed that a few of SCoT primers might be sufficient for rapid identification of registered varieties.SCoT molecular markers might play a role in protecting interest of farmers and breeders and promote the healthy development of sugar industry in China.
作者
邳植
吴则东
黄林玉
Pi Zhi;Wu Zedong;Huang Linyu(College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080)
出处
《中国糖料》
2020年第4期36-39,共4页
Sugar Crops of China
基金
国家现代农业(糖料)产业技术体系“甜菜高品质品种改良岗位专项基金”(CARS-170111)
黑龙江省省属高等学校基本科研业务费基础研究项目“基于共表达网络解析甜菜春化分子调控机制的研究”(RCCXYJ201810)资助。
关键词
甜菜
品种鉴定
指纹图谱
SCoT
分子标记
sugar beet
variety identification
fingerprint
SCoT
molecular marker
