摘要
为了分析青海西宁地区猪肉食品中沙门菌致病性、血清型及耐药性流行情况,从青海西宁地区屠宰场、零售店、超市等采集猪肉食品样品246份进行沙门菌分离鉴定,采用人工感染小鼠试验、Kauffmann-White法、K-B药敏纸片法分别检测沙门菌的致病性、血清型及耐药性。结果显示,分离得到117株沙门菌中56株对小鼠具有致病性;分离的56株致病性沙门菌属于10种血清型,以猪霍乱沙门菌、鼠伤寒沙门菌和肠炎沙门菌3种血清为主要流行致病血清型,分别占致病菌株的26.8%、21.4%和19.6%;分离的56株致病性沙门菌分离株对氨苄西林、新霉素、阿莫西林等9种药物耐药率在50%以上,对其他药物耐药率在12.5%~35.7%。表明青海西宁地区猪肉食品中分离沙门菌血清型较为复杂,且耐药性严重。
In order to analyze the pathogenicity,serotype and drug resistance of Salmonella in pork food in Xining region of Qinghai.246 pork samples were collected from slaughterhouses,retail stores and supermarkets in Xining region of Qinghai province to isolate and identify Salmonella.The pathogenicity,serotype and drug resistance of Salmonella were detected by artificial infection of mice,Kauffmann white method and K-B drug sensitive paper method.The results showed that 56 of 117 Salmonella strains were pathogenic to mice,and 56 of them belonged to 10 serotypes of which Salmonella cholerae,Salmonella typhimurium and Salmonella enteritidis were the main epidemic serotypes,accounting for 26.8%,21.4% and 19.6% of the pathogenic strains,respectively.56 of them were resistant to 9 drugs,including ampillin mycomycin and amoxicillin,and the resistant rate was more than 50%,and the drug resistance rate of other drugs was between 12.5% and 35.7%.The results showed that the serotypes of Salmonella isolated from pork food in Xining region of Qinghai were complex and drug resistant.
作者
赵维章
张正英
ZHAO Wei-zhang;ZHANG Zheng-ying(Animal Disease Prevention and Control Center of Qinghai Province,Xining 810000,China)
出处
《中国兽医杂志》
CAS
北大核心
2020年第4期80-82,86,共4页
Chinese Journal of Veterinary Medicine
关键词
猪肉食品
沙门菌
致病性
血清型
耐药性
pork food
Salmonella
pathogenicity
serotype
drug resistance
