摘要
从福建省莆田市仙游县游洋镇采集患有疑似炭疽病的甜柿叶片,按照常规方法进行病原菌的分离纯化,通过形态学特征和ITS-TUB2-CAL-ACT基因构建的多基因系统发育树对获得的菌株进行鉴定,并经致病性测定,确定分离到的菌株为胶孢炭疽复合种(the Colletotrichum gloeosporioides species complex)中的果生刺盘孢(Colletotrichum fructicola).药剂筛选结果显示,不同药剂对该菌的抑制效果存在一定差异.其中,75%百菌清对该菌菌丝生长和孢子萌发的抑制活性最强,抑制率分别为71.64%和85.69%.
Sweet persimmon leaves from Xianyou County,Putian City,Fujian Province,which were suspected to be infected with anthracnose were collected to isolate and purify the pathogen by conventional methods.The strains were identified as Colletotrichum fructicola from the C.gloeosporioides species complex by morphological characteristics,multiple gene phylogenetic tree based on ITSTUB2-CAL-ACT,and pathogenicity detection.The fungicides screening showed that different fungicides displayed various inhibitory effects on C.fructicola.Among them,75%chlorothalonil showed the best inhibitory activities on mycelium growth and conidia germination with the inhibition rate being 71.64%and 85.69%,respectively.
作者
张童
姚立萍
胡玉慈
唐鑫彪
何淑敏
陈清西
文志丰
ZHANG Tong;YAO Liping;HU Yuci;TANG Xinbiao;HE Shumin;CHEN Qingxi;WEN Zhifeng(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fuzhou Institute of Agricultural Science,Fuzhou,Fujian 350018,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2020年第5期589-596,共8页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家自然科学基金(青年)项目(31701907)
福建省自然科学基金(高校联合)项目(2018J01703)
福建农林大学杰青项目(xjq201723)
福建省教育厅中青年教师教育科研项目(JAT170163)
福建省现代农业产业技术体系建设专项【闽财指[2017]625号】
中央引导地方科技发展专项(2017L3001).
关键词
柿炭疽病
病原鉴定
多基因序列分析
果生刺盘孢
室内毒力测定
persimmon anthracnose
pathogenic identification
multiple gene sequence analysis
Colletotrichum fructicola
indoor toxicity measurement
