摘要
为探索树莓最佳的组织培养快速繁殖技术,以新生的腋芽为外植体,以MS为基础培养基,加入不同浓度的细胞分裂素6-BA、生长素NAA及IBA,研究树莓的离体快繁技术。结果表明:最佳的初代培养基是1/2 MS+6-BA 0.1 mg·L^-1+NAA 0.15 mg·L^-1+1.0 g·L^-1活性炭;增殖最佳培养基为MS+6-BA 0.5 mg·L^-1+NAA 0.01 mg·L^-1,增殖系数为4.7。最佳的生根培养基为1/2 MS+IBA 0.8 mg·L^-1+1.0 g·L^-1活性炭,生根率达到100%,平均的根条数是9条,平均的根长是7.6 cm。
In order to explore the best tissue culture and rapid propagation technology of raspberry,new axillary buds were used as explants,MS as basic medium,and different concentrations of cytokinin 6-BA,auxin NAA and IBA were added.The results showed that the best primary culture medium was 1/2 MS+6-BA 0.1 mg·L^-1+NAA 0.15 mg·L^-1+activated carbon 1.0 g·L^-1;the best medium for proliferation was MS+6-BA 0.5 mg·L^-1+NAA 0.01 mg·L^-1,and the proliferation coefficient was 4.7.The best rooting medium was 1/2 MS+IBA 0.8 mg·L^-1+1.0 g·L^-1 activated carbon,the rooting rate reached 100%,the average number of roots was 9,and the average root length was 7.6 cm.This method can be used for rapid propagation of raspberry.
作者
徐亚英
黄晓梅
罗育
谢丽
XU Ya-ying;HUANG Xiao-mei;LUO Yu;XIE Li(Heilongjiang Agricultural Vocational and Technical College,Jiamusi 154007,China)
出处
《黑龙江农业科学》
2020年第9期70-74,共5页
Heilongjiang Agricultural Sciences
基金
黑龙江农业职业技术学院校级大学生创新创业项目。
关键词
树莓
初代培养
增殖培养
生根培养
raspberry
primary culture
proliferation culture
rooting culture
