摘要
以5个优良树莓栽培品种为试验材料，建立了树莓茎尖组织培养体系。结果表明：原初培养采用培养基MS＋0．35mg／LBA＋0．1mg／L GA1，腋芽萌发率可达85％以上；树莓品种‘早红’和‘美22’增殖培养基采用MS＋0．3mg／L GA3＋0．1mg／L IAA＋0．25mg／L BA＋琼脂0．5％，‘澳洲红’采用MS＋0．3mg／L GA3＋0．3mg／L IAA＋0．25mg／L BA＋琼脂0．5％，‘黄树莓’采用MS＋0．3mg／L GA3＋0．3mg／LIAA＋0．25mg／LBA＋琼脂0．3％，‘黑莓A4-17’采用MS＋0．3mg／L GA3＋0．3mg／L IAA＋0．35mg／L BA＋琼脂0．5％，其增殖系数可达5～10；生根培养基采用1／2MS＋1．00mg／L IBA，生根率可达70％～90％；移栽适应性培养以河沙作为移栽基质最佳，成活率可达80％以上，但不同品种间有一定差异。
In the present experiment, five raspberry varieties were employed as the experimental materials, the rapid propagation system based on shoot-tip culture had been established. It was found that： the most suitable cultural medium for germination is MS＋0.35 mg/L BA＋0.1 mg/L GA3 ,germinating rate could reach more than 85 % with the medium. The best media for multiplication are followings： ‘Early-ripening red＇ raspberry and ‘American No. 22＇ with MS＋0.3 mg/L GA3 ＋0.1 mg/L IAA＋0.25 mg/L BA＋agar 0.5%;‘Australia red＇ raspberry with MS＋0. 3 mg/L GA3＋0. 3 mg/L IAA＋0. 25 mg/L BA＋agar 0.5%;＇Yellow raspberry＇ with MS＋0. 3 mg/L GA3＋0. 3 mg/L IAA＋0. 25 mg/L BA＋agar 0. 3%; ‘ Blackberry A4-17＇ with MS＋ 0.3 mg/L GA3 ＋ 0. 3 mg/L IAA＋ 0.35 mg/L BA＋ agar 0.5 %, propagation coefficient could reach 5-10 times. Rooting rate could reach 70%-90% with the medium of 1/2 MS＋ 1.00 mg/L IBA. Transplanting surviving rate could reach more than 80% on the river sand.
出处
《西北植物学报》
CAS
CSCD
北大核心
2007年第9期1892-1898,共7页
Acta Botanica Boreali-Occidentalia Sinica
关键词
树莓
组织培养
体系优化
raspberry
tissue culture
system optimization
