摘要
目的采用1H-NMR代谢组学技术评价何首乌炮制品中的差异代谢物。方法建立何首乌的1H-NMR指纹图谱,对黑豆汁制、黄酒制、清水制、黑豆汁加黄酒制何首乌以及生首乌进行分析,同时考察6、12、18、24、32、36 h炮制时间,采用主成分分析、偏最小二乘法判别分析等多元统计方法对不同炮制品与生品差异进行比较分析。结果在何首乌生品、炮制品中找到15种差异化学成分,其中苏氨酸、脯氨酸、没食子酸、β-葡萄糖、琥珀酸、乳酸、胆碱、甘氨酸含量具有随着炮制而升高趋势;而γ-氨基丁酸、蔗糖具有降低的趋势;二苯乙烯苷的含量是先升高后降低。结论基于NMR技术的代谢指纹图谱可以较好的评价何首乌的不同炮制工艺。
Objective To investigate the significant changed metabolites in processed products of Polygoni Multiflori Radix based on fingerprint of 1H-NMR.Methods 1H-NMR fingerprint were established.Four processing methods(black bean juice,yellow rice wine,clean water,and black bean juice plus yellow rice wine)and six processing time points(6,12,18,24,32,and 36 h)of Polygoni Multiflori Radix were analyzed.PCA and OPLS-DA analysis were utilized to distinguish the difference among the processed Polygoni Multiflori Radix and crude Polygoni Multiflori Radix.Results 15 Distinguished compounds were found between crude Polygoni Multiflori Radix and different processed Polygoni Multiflori Radix.The tendency of threonine,proline,gallic acid,β-glucose,succinate,lactate,choline,and glycine were increased along with processing time.However,γ-aminobutyrate and sucrose were decreased significantly.The content of stilbene glycoside was increased at first and decreased finally.Conclusion The 1H-NMR fingerprint could be better used for accessing the processing technologies of Polygoni Multiflori Radix.
作者
王健
孙瑜
陈莉莉
韩真真
孙精通
WANG Jian;SUN Yu;CHEN Li-li;HAN ZHen-zhen;SUN Jing-tong(Tianjin Children’s Hospital,Tianjin 300074,China;Tianjin University of TCM,Tianjin 301617,China)
出处
《现代药物与临床》
CAS
2020年第8期1537-1543,共7页
Drugs & Clinic
关键词
何首乌
差异代谢物
炮制
核磁共振氢谱
指纹图谱
Polygoni Multiflori Radix
changed metabolites
processing technology
1H-NMR
fingerprint
