摘要
目的:探讨桔梗多糖对OVA诱导的哮喘小鼠气道炎症和气道重塑的影响。方法:将60只小鼠随机分成6组,每组10只,即正常组、模型组、桔梗多糖(PGP)低、中、高剂量(20、60、80 mg/kg)组和阳性对照地塞米松组。采用卵白蛋白致敏的方法建立哮喘模型后,各组分别予以相应药物干预8周。给药结束后,测定小鼠气道高反应性。分别取材,用于ELISA检测、HE染色、AB-PAS染色和吉姆萨染色计数,Western blot检测肺组织中气道重塑相关蛋白转化生长因子-β1(TGF-β1)、平滑肌肌动蛋白(α-SMA)和周期蛋白(Cyclin D1、Cyclin D2)的蛋白表达变化。结果:与正常组相比,模型组小鼠肺阻力(RL)显著升高,动态顺应性(Cdyn)显著降低,BALF中Eotaxin(嗜酸细胞活化趋化因子)、IL-4、IL-5、IL-13和血清中IgE显著升高,肺组织中炎症细胞浸润和黏液分泌明显增多,支气管杯状细胞增生明显,气道重塑相关蛋白表达显著升高;桔梗多糖高、中、低剂量组均能降低小鼠RL,升高Cdyn,降低BALF中Eotaxin、IL-4、IL-5、IL-13和血清IgE的含量,明显减少肺组织中炎症细胞的产生、黏液的分泌和杯状细胞的增生以及气道重塑相关蛋白的表达;且桔梗多糖高剂量组与阳性对照地塞米松组比,无统计学差异。结论:桔梗多糖能够降低哮喘小鼠的气道高反应性,减轻炎性反应,降低血清IgE水平,同时能够降低肺组织TGF-β1、α-SMA、Cyclin D1和Cyclin D2蛋白表达,延缓气道重塑。
Objective:To investigate the effects of Platycodon grandiflorum polysaccharide(PGP)on airway inflammation and remodeling in OVA-induced asthmatic mice.Methods:60 healthy BALB/c mice were randomly divided into six groups:normal group,asthmatic model group,PGP low,medium and high-dose(20,60,80 mg/kg)groups and Dexamethasone positive group of 10 mice.After establishment of asthma model by ovalbumin sensitization,each group was given corresponding medicine.The airway hyperreactivity in asthma mice was measured after administration,the concentrations of inflammatory cytokine were measured by enzyme-linked immunoassays(ELISA),and morphological abnormality and tissue inflammation were determined through HE staining and AB-PAS staining and inflammatory cells were counted by Jimsa staining.Lung tissue was collected for Western blot analysis of expression of transforming growth factor-beta 1(TGF-β1),smooth muscle actin(α-SMA)and cyclin D1,Cyclin D2.Results:Compared with the normal group,the Lung Resistance(RL)were significantly increased while the dynamic compliance(cdyn)were significantly decreased in the model group,levels of Eotaxin,IL-4,IL-5,IL-13 in BALF and IgE in serum of model group were significantly increased,inflammatory cell infiltration and mucus secretion in lung tissue increased significantly in model group while goblet cell proliferation in bronchus was visible,the expression of airway remodeling correlated proteins was significantly increased in model group.PGP low,medium and high-dose groups could reduce the RL and increase t cdyn in mice,the levels of Eotaxin,IL-4,IL-5,IL-13 in BALF and IgE in serum were decreased in PGP low,medium and high-dose groups,the production of inflammatory cells,the secretion of mucus,the proliferation of goblet cells and the expression of airway remodeling related proteins were significantly reduced;there was no significant difference between the PGP high dose group and the Dxamethasone group.Conclusion:PGP may inhibit asthmatic airway inflammation and airway hyperreactivity
作者
姚欢欢
陈吉
李岚
YAO Huanhuan;CHEN Ji;LI Lan(Third People’s Hospital of Huzhou,Huzhou,Zhejiang 313002;First Affiliated Hospital of Zhejiang University of Chinese Medicine,Hangzhou,Zhejiang 310006)
出处
《中国中医药科技》
CAS
2020年第5期701-707,共7页
Chinese Journal of Traditional Medical Science and Technology
基金
浙江省自然科学基金资助项目(LY15H270006)。
