摘要
目的探讨喉癌组织中轴突导向因子3D(SEMA3D)的表达与患者临床病理特征和预后的关系。方法收集2015年1月—2016年12月经外科手术切除的喉癌组织及对应癌旁组织标本61例,应用免疫组化SP法检测SEMA3D的表达情况,并对SEMA3D与喉癌临床病理特征及预后的关系进行统计学分析。结果免疫组化SP法显示,SEMA3D在喉癌组织中的表达水平显著低于在癌旁正常组织中的表达水平(P<0.05)。SEMA3D表达与患者的临床分期和颈部淋巴结转移密切相关(P<0.05)。Kaplan-Meier生存分析显示,SEMA3D表达与患者生存率具有相关性(P<0.001)。单变量Log-rank检验分析显示吸烟、临床分期、颈部淋巴结转移和SEAM3D表达与患者预后具有相关性,进一步行Cox多因素回归分析发现SEMA3D低表达是影响喉癌预后的独立危险因素。结论SEMA3D蛋白低表达是影响喉癌预后的独立危险因素。SEMA3D蛋白阳性表达的喉癌患者预后较好,有望成为喉癌患者预后评估的新的生物标志物。
Objective To investigate the expression of Semaphporin 3 D in laryngeal cancer tissue and its relationship with clinicopathological characteristics and prognosis of patients.Methods Sixty-one laryngeal cancer tissues and normal tissues adjacent to carcinoma were obtained from January 2015 to December 2016.Immunohistochemistry SP staining was used to detect the expression of Semaphporin 3 D.The relationships of its expression with clinicopathological characteristics and prognosis were analyzed.Results Immunohistochemistry SP method showed that SEMA3 D expression was significantly lower in laryngeal cancer tissue than in normal tissue adjacent to carcinoma(P<0.05).The expression of SEMA3 D was closely related to clinical stage and lymph node metastasis(P<0.05).Kaplan-Meier survival analysis showed that SEMA3 D expression was correlated with patient survival(P<0.001).Univariate Log-rank test showed that smoking,clincial staging,lymphnode metastasis and expression of SEMA3 D were associated with the prognosis of patients.Multivariate Coxregression analysis further showed that the low expression of SEMA3 D was an independent risk factor affecting the prognosis of laryngeal cancer.Conclusions Low expression of SEMA3 D protein is an independent risk factor for the prognosis of laryngeal cancer.Laryngeal cancer patients with positive SEMA3 D expression show better prognosis than those with negative SEMA3 D expression,which indicates that SEMA3 D may be a potential biomarker for the prediction of prognosis in patients with laryngeal cancer.
作者
吴俊志
李静佳
赵锐
朱鑫
冯成敏
敬一丹
程瑶
张震
邓世山
刘海
WU Junzhi;LI Jingjia;ZHAO Rui;ZHU Xin;FENG Chengmin;JING Yidan;CHENG Yao;ZHANG Zhen;DENG Shishan;LIU Hai(Department of Otolaryngology Head and Neck Surgery,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China;Department of Anatomy,North Sichuan Medical College,Nanchong 637000,China)
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2020年第4期416-420,共5页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
四川省教育厅高校创新团队项目(17TD0016)
川北医学院校级课题(CBY17-A-YB56)。
关键词
喉癌
SEMA3D
回归分析
免疫组化
预后
Laryngeal cancer
SEMA3D
Regression analysis
Immunohistochemistry
Prognosis
