摘要
目的:探讨桑叶生物碱改善小鼠蛋白氧化损伤作用效果与机理,旨在为桑叶生物碱的开发利用提供理论依据。方法:采用D-半乳糖(D-galactose,D-Gal)诱导建立小鼠氧化损伤模型,灌胃不同剂量的桑叶生物碱,于第8周末测定小鼠血浆蛋白羰基(protein carbonyl,PCO)、晚期蛋白氧化产物(advanced oxidation protein products,AOPP)、3-硝基酪氨酸(3-nitrotyrosine,3-NT)含量及超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、醌氧化还原酶1(NAD(P)H quinine oxidoreductase 1,NQO1)活力,实时荧光定量聚合酶链式反应测定肝脏组织SOD、GSH-Px、NQO1、核因子E2相关因子2(nuclear erythroid related factor 2,Nrf2)、Kelch样环氧氯丙烷相关蛋白1(Kelch like ECH-associated protein-1,Keap1)mRNA表达情况。结果:与模型对照组相比,桑叶生物碱高剂量组(200 mg/kg mb)小鼠血浆中PCO、AOPP、3-NT水平分别降低36.43%、61.81%、58.13%(P<0.01);血浆中的SOD、GSH-Px、NQO1活力分别提高48.89%、167.17%、85.12%(P<0.01)。同时,桑叶生物碱高剂量组肝脏中SOD1、SOD2、GSH-Px、NQO1、Nrf2 mRNA相对表达量分别增加96.96%、94.26%、116.71%、101.51%、63.01%(P<0.01),Keap1 mRNA相对表达量下降33.54%(P<0.01)。结论:桑叶生物碱可能通过调控Nrf2/Keap1氧化应激信号通路实现对D-Gal诱导的小鼠蛋白氧化损伤的保护作用。
Objective:To explore the effect of alkaloids from mulberry leaves on improving oxidative protein damage in mice and the underlying mechanism for the purpose of providing a theoretical basis for the development and utilization of alkaloids from mulberry leaves.Methods:A mouse model of oxidative damage was induced by D-galactose(D-Gal)and administered with different doses of mulberry leaf alkaloids.At the end of the 8 th week,the contents of protein carbonyl(PCO),advanced oxidation protein products(AOPP)and 3-nitrotyrosine(3-NT),and the enzyme activity levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and NAD(P)H quinine oxidoreductase 1(NQO1)in plasma were measured.The mRNA expression of SOD,GSH-Px,NQO1,nuclear erythroid related factor 2(Nrf2)and Kelchlike ECH-associated protein-1(Keap1)in liver tissues were also detected by real-time polymerase chain reaction(qPCR).Results:Compared with the model control group,the plasma PCO,AOPP and 3-NT levels of mice decreased by 36.43%,61.81%and 58.13%,respectively after intragastric administration of high-dose mulberry leaf alkaloids(200 mg/kg mb)(P<0.01).However,the activity of SOD,GSH-Px and NQO1 in plasma increased by 48.89%,167.17%and 85.12%(P<0.01).Meanwhile,high-dose mulberry leaf alkaloids also increased the mRNA expression of SOD1,SOD2,GSH-Px,NQO1 and Nrf2 in the liver by 96.96%,94.26%,116.71%,101.51%and 63.01%,respectively(P<0.01),but decreased the mRNA level of Keap1 by 33.54%(P<0.01).Conclusion:Mulberry leaf alkaloids have a protective effect on D-Gal-induced oxidative protein damage in mice via adjusting Keap1/Nrf2 signal pathway.
作者
杨忠敏
沈以红
黄先智
王祖文
丁晓雯
YANG Zhongmin;SHEN Yihong;HUANG Xianzhi;WANG Zuwen;DING Xiaowen(Chongqing Key Laboratory of Agricultural Products Processing,National Demonstration Center for Experimental Food Science and Technology Education,College of Food Science,Southwest University,Chongqing 400716,China;Science and Technology Department,Southwest University,Chongqing 400716,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第17期182-187,共6页
Food Science
基金
现代农业产业技术体系建设专项(CARS-18)。
