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矿泉水中铜绿假单胞菌的测定能力验证结果分析 被引量:10

Proficiency testing results analysis of determination of Pseudomonas aeruginosa in mineral water
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摘要 目的分析矿泉水中铜绿假单胞菌检测能力验证(ACAS-PT 736(2019))的结果。方法根据能力验证指导书,并参照GB 4789.2-2016《食品安全国家标准食品微生物学检验菌落总数测定》对样品进行稀释,依据GB 8538-2016《食品安全国家标准饮用天然矿泉水检验方法》对可疑菌落进行鉴定,同时对可疑菌落使用VITEK2进行辅助鉴定。结果样品19-P383检测结果为7800 CFU/250 mL,样品19-Q333检测结果为8800 CFU/250 mL。本次能力验证Z值分别为-0.8、-0.1、|Z|均小于2,检测结果满意。结论本实验室具备检测铜绿假单胞菌的能力, VITEK2对可疑菌落进行辅助鉴定的准确性也得到了验证。 Objective To analyze the results of the proficiency testing(ACAS-PT 736(2019) of Pseudomonas aeruginosa in mineral water. Methods Samples were diluted according to the proficiency test guidance and GB 4789.2-2016 National food safety standard-Food microbiological inspection total colony determination. Suspicious colonies were identified according to GB 8538-2016 National food safety standard-Test method for drinking natural mineral water, and VITEK2 was used for auxiliary identification of suspicious colonies at the same time. Results The test result of sample 19-P383 was 7800 CFU/250 mL, and the test result of sample 19-Q333 was 8800 CFU/250 mL. The test results were satisfactory as the Z values of the proficiency test were -0.8, -0.1, and |Z| were less than 2. Conclusion This laboratory has the ability to detect Pseudomonas aeruginosa in mineral water, and the accuracy of VITEK2’ s auxiliary identification of suspicious colonies has been verified.
作者 韦云 陈佩虹 周露 WEI Yun;CHEN Pei-Hong;ZHOU Lu(Guangdong Institute of Food Inspection,Guangzhou 510435,China)
出处 《食品安全质量检测学报》 CAS 2020年第16期5398-5402,共5页 Journal of Food Safety and Quality
基金 国家市场监督管理总局科技计划项目(2019MK057)。
关键词 能力验证 铜绿假单胞菌 VITEK2 矿泉水 proficiency testing Pseudomonas aeruginosa VITEK2 mineral water
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