摘要
目的探讨长链非编码RNA FENDRR对前列腺癌DU-145细胞生物学行为的影响。方法 realtime PCR方法检测前列腺癌细胞株DU-145和正常前列腺上皮细胞RWPE-1中FENDRR的表达水平。构建FENDRR基因表达载体pcDNA-FENDRR并转染DU-145细胞,real-time PCR方法验证转染效率。FENDRR基因过表达后,平板克隆实验实验检测DU-145细胞增殖能力的变化;流式细胞术检测DU-145细胞的凋亡率;划痕实验与Transwell实验检测DU-145细胞迁移和侵袭能力的变化。结果 FENDRR在前列腺癌细胞株DU-145中的表达明显低于正常前列腺上皮细胞RWPE-1(P<0.01)。pcDNA-FENDRR转染显著上调DU-145细胞中FENDRR mRNA的表达。FENDRR基因过表达后,DU-145细胞的增殖、迁移与侵袭能力明显降低,细胞凋亡率明显升高(P<0.01)。结论过表达lncRNA FENDRR能够抑制前列腺癌DU-145细胞增殖、迁移与侵袭,并促进凋亡,在前列腺癌中发挥肿瘤抑制基因的作用。
Objective To investigate the effect of long-chain noncoding RNA FENDRR on the biological behavior of prostate cancer DU-145 cells. Methods The expression of FENDRR in prostate cancer cell line DU-145 and normal prostate epithelial cell line RWPE-1 was detected by real-time PCR. FENDRR gene expression vector pcdnaFENDRRwas constructed and transfected into DU-145 cells, the transfection efficiency was verified by real-time PCR method. After the overexpression of FENDRR gene, the proliferation ability of DU-145 cells was detected by plate cloning experiment;the apoptosis rate of DU-145 cells was detected by flow cytometry;the migration and invasion ability of DU-145 cells was detected by scratch experiment and Transwell experiment. Results The expression of FENDRR in DU-145 was significantly lower than that in RWPE-1(P<0.01). Transfection of pcdna-FENDRRcould upregulated significantly the level of FENDRR mRNA in DU-145 cells. After overexpression of FENDRR gene, the proliferation, migration and invasion ability of DU-145 cells was decreased significantly, and the apoptosis rate was increased significantly(P<0.01). Conclusion Overexpression of lncRNAFENDRR could inhibit the proliferation, migration and invasion of DU-145 cells, promote apoptosis, and play the role of tumor suppressor gene in prostate cancer.
作者
金玮
宋彦
王侠
费翔
陈芳杰
JIN Wei;SONG Yan;WANG Xia;FEI Xiang;CHEN Fang-jie(Department of Urology,Shengjing Hospital of China Medical University,Shenyang 110004;Department of Medical Genetics,School of Life Sciences,China Medical University,Shenyang 110122,China)
出处
《解剖科学进展》
2020年第4期434-436,441,共4页
Progress of Anatomical Sciences
基金
辽宁省教育厅科学研究项目(L2015587)。
