摘要
对出现维管束变色、植株矮化且叶片变黄的秋葵植株茎秆应用组织分离法进行分离、纯化,获得单孢菌株。用孢子悬浮液接种水培秋葵幼苗进行致病性测定,并通过形态学观察及分子生物学对病原菌进行鉴定。结果表明,供试秋葵菌株的菌落特征,分生孢子、分生孢子梗和微菌核形态均与大丽轮枝菌(Verticillium dahliae)一致;经分子生物学鉴定,供试菌株的ITS-ACT基因序列与大丽轮枝菌(V.dahliae,登录号:HQ206859和HQ206856,HQ206975和HQ206966)相似性达99.5%以上。因此,将引起石河子地区秋葵黄萎病的病原菌鉴定为大丽轮枝菌(Verticillium dahliae),且非落叶型和落叶型菌系均能侵染秋葵。
The stems of okra plants with discolored vascular bundles,dwarfized plants and yellowing leaves were isolated and purified by tissue separation to obtain monospora strains.Pathogenicity of isolations was tested with spore suspension inoculating water culture okra seedling,and the pathogen was identified based on morphological characteristics and sequence analysis of rDNA-ITS region and actin gene.The results showed that the colony morphology,conidia,conidiophore and microsclerotium of the strains were consistent with Verticillium dahliae.The ITS-ACT gene sequence of the tested strains showed 99.5%sequence similarity with V.dahliae(accession numbers:HQ206859 and HQ206856,HQ206975 and HQ206966).Therefore,the pathogen of Verticillium Wilt of Okra was identified as Verticillium dahliae,non-deciduous and deciduous type strains can infect okra in Shihezi area.
作者
孙璘
王成成
李国英
李番
焦瑞莲
任毓忠
SUN Lin;WANG Chengcheng;LI Guoying;LI Fan;JIAO Ruilian;REN Yuzhong(College of Agriculture,Shihezi University,Shihezi Xinjiang 832003,China;Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization,Xinjiang Uygur Autonomous Region,Shihezi Xinjiang 832003,China)
出处
《甘肃农业科技》
2020年第8期22-27,共6页
Gansu Agricultural Science and Technology
基金
国家自然科学基金项目(31160357)
科技部中小企业创新项目(14C26216513812)
国家重点资助研发项目(2017YFD0201900)。
关键词
秋葵
黄萎病
多基因联合
病原鉴定
石河子
Okra
Verticillium wilt
Polygenic association
Pathogen identification
Shihezi
