摘要
目的基于毒素多肽建立一种新型的全蝎质量检测工艺。方法从东亚钳蝎毒液中分离得到了一种新型钠通道毒素BmK NaTx-13,制备并鉴定了BmK NaTx-13的特异性抗体,并通过竞争性ELISA法检测全蝎提取物中的BmK NaTx-13的含量。结果建立的竞争性酶联免疫吸附法可快速检测全蝎提取物中的BmK NaTx-13的含量并呈现良好的浓度线性依赖。结论该研究为全蝎质控提供了一种快速免疫检测方法,为提升全蝎入药质量标准提供了借鉴。
OBJECTIVE To develop a BmK Scorpion quality control technology based on toxin peptide.METHODS In this study,a novel sodium channel toxin BmK NaTx-13 was isolated from BV,and the specific polyclonal antibody against BmK NaTx-13 was obtained by immunizing rabbits.RESULTS A competitive enzyme linked immunosorbent assay(ELISA)based on BmK NaTx-13 specific antibody could detect the content of BmK NaTx-13 in the processed BmK scorpion extract and showed a good linear concentration dependent.CONCLUSION This research provided a rapid immunoassay method for establishing the quality detection processed BmK scorpion,and provided an insight for improving the quality standard of whole scorpion medicine.
作者
陈永根
桑明
卢悟广
王明远
刘金满
陈胜君
李松
胡春萍
王奇
曹鹏
CHEN Yong-gen;SANG Ming;LU Wu-guang;WANG Ming-yuan;LIU Jin-man;CHEN Sheng-jun;LI Song;HU Chun-ping;WANG Qi;CAO Peng(Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China;Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China;Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210028, China;School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China;Jiangsu Key Laboratory of Key Technology for Preparation and Quality Control of Traditional Chinese Medicine Formula Particles, Jiangyin Tianjiang Pharmaceutical Co., Ltd, Wuxi, 214400, China)
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2020年第4期509-516,共8页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家自然科学基金优秀青年基金(81622048)
广州市科技局项目(201805010005)。
