摘要
目的探讨新型冠状病毒(SARS-CoV-2)核酸检测在发热门诊、隔离病房、易感人群以及大规模筛查等不同人群中的应用价值,为SARS-CoV-2实验室检测提供参考依据。方法采集2020年2月1日—3月22日郑州人民医院不同人群的咽拭子,包括发热门诊、隔离病房患者,一线疫情防控医护人员,以及无新型冠状病毒肺炎(COVID-19)症状的住院患者、陪护家属和复工人员。采用逆转录聚合酶链反应(RT-PCR)方法,使用A、B两种国产检测试剂盒对发热门诊、隔离病房的患者进行SARS-CoV-2核酸检测。结果共检测15497份咽拭子标本,SARS-CoV-2核酸阳性24份,核酸阳性病例均来自发热门诊和隔离病房。24例咽拭子SARS-CoV-2核酸阳性患者同时送检的血标本核酸结果均呈阴性。A、B两种试剂首次咽拭子核酸检测结果一致,2例确诊COVID-19患者治疗后复查咽拭子标本,A组试剂检测为阳性,B组试剂检测为阴性。该院疫情防控一线医护人员、未出现COVID-19症状的住院患者、陪护家属和复工体检人员核酸检测均为阴性。结论复工人员、住院患者、陪护家属和一线疫情防控人员SARS-CoV-2核酸筛查均未发现阳性,咽拭子标本SARS-CoV-2核酸检测阳性率高于血标本,不同核酸检测试剂检测阳性率稍有差异。
Objective To explore the application value of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)nucleic acid test in different population,including persons in fever clinic and isolation wards,susceptible persons as well as persons in large-scale screening,provide reference for laboratory nucleic acid test of SARS-CoV-2.Methods Laryngeal swabs from different population in People’s Hospital of Zhengzhou between February 1,2020 and March 22,2020 were collected,including patients in fever clinic and isolation wards,anti-epidemic front-line health care workers(HCWs),hospitalized patients without symptoms of coronavirus disease 2019(COVID-19),accompanying family members,and persons returning to work.Real-time reverse transcriptase-polymerase chain reaction(RT-PCR)and two domestic detection kits A and B were used to test SARS-CoV-2 nucleic acid in patients in fever clinic and isolation wards.Results A total of 15497 laryngeal swabs were detected,24 of which were positive for SARS-CoV-2 nucleic acid test,nucleic acid test positive cases were all from fever clinic and isolation wards.24 cases of SARS-CoV-2 nucleic acid test positive laryngeal swabs were all negative for blood specimens tested at the same time.The first laryngeal swab test results of kits A and B were identical,re-test of 2 confirmed cases after treatment were positive in kit A group and negative in kit B group.Nucleic acid test results were all negative in front-line HCWs,hospitalized patients without COVID-19 symptoms,accompanying family members and physical examination persons returning to work.Conclusion No positive results are found in the screening among the returning workers,hospitalized patients,accompanying family members and front-line epidemic prevention and control professionals,positive rate of SARS-CoV-2 nucleic acid of laryngeal swab specimens is higher than that of blood specimens,positive rates of different nucleic acid test reagents are slightly different.
作者
胡亚会
郑阳
李莹莹
陈军
孟婧洁
闫琛
宋银森
HU Ya-hui;ZHENG Yang;LI Ying-ying;CHEN Jun;MENG Jing-jie;YAN Chen;SONG Yin-sen(Translational Medicine Research Center, People’s Hospital of Zhengzhou, Zhengzhou 450015, China)
出处
《中国感染控制杂志》
CAS
CSCD
北大核心
2020年第9期806-811,共6页
Chinese Journal of Infection Control
基金
河南省医学科技攻关计划联合共建项目(2018020824)。
关键词
新型冠状病毒
逆转录聚合酶链反应
核酸检测
咽拭子
coronavirus disease 2019
real-time reverse transcriptase-polymerase chain reaction
nucleic acid test
laryngeal swab
