摘要
目的探讨SARS患者和康复者的血清学和病原学变化;筛选SARS康复者淋巴细胞中差异表达的基因。方法ELISA、冠状病毒全基因组芯片、抑制消减杂交(SSH)。结果80.4%的SARS康复者和2%的密切接触者SARSCoV的IgG抗体为阳性,康复后8个月内康复者抗体平均水平呈下降趋势;芯片能检测出血、便、痰和尿标本中的冠状病毒核酸,发现编码S1蛋白的核酸在大部分标本中是连续的;SSH获得了SARS康复者淋巴细胞中差异表达的77个cDNA克隆,其中4个为未知的新序列。结论大多数SARS康复者产生了冠状病毒特异的IgG抗体;密切接触者中可能存在隐性感染者;冠状病毒全基因组芯片能检测出各标本中的病毒核酸并建立相应的基因谱;SSH文库为筛选新型冠状病毒特异的抗体基因奠定了分子基础。
OBJECTIVE To detect the dynamics of serology and pathogenesis of SARS virus in the SARS patients and convalescent persons, and screen the differentially expressed genes of lymphocyte in the convalescent SARS persons. METHODS ELISA, whole genomic gene chip of coronavirus, and SSH were used. RESULTS IgG antibody was found in 80.4% convalescent SARS persons and 2% close contacters, the average IgG antibody level declined in the eight months. The nucleic acid of coronavirus in the specimen of blood, feces, sputum and urine was detected with gene chip, the sequence that code S1 protein was more continuous in most specimens was compared with other sequences. SSH result showed that total 77 different clones were gotten in the lymphocyte of convalescent SARS patients, and 4 clones might represent novel genes. CONCLUSIONS Most of the convalescent persons from SARS could generate specific IgG antibody; the recessive infected persons may be existed among the close contacters; the whole genomic chip of coronavirus can be used to detect the coronavirus′s nucleic acid and establish their corresponding gene profile. The SSH library provides a basis for further screen the series genes that express the SARS coronavirus specific IgG antibodies.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2005年第1期1-4,共4页
Chinese Journal of Nosocomiology
基金
国家863基金NO.2003AA208107
关键词
基因芯片
SARS
IGG
康复
Gene chip
SARS
Immunoglobulins
Convalescence
