摘要
目的研究载银二氧化钛(Ag-Ti O2)对白色念珠菌生物被膜的抑制作用,并初步探讨其作用机制及靶点。方法通过XTT法检测Ag-Ti O2对白色念珠菌生物被膜的影响并测定其黏附最低抑菌浓度(SMIC);在倒置显微镜下观察不同浓度Ag-Ti O2作用后白色念珠菌菌丝生长状况,考察Ag-Ti O2对白色念珠菌酵母态向菌丝态转变的影响;使用水-烃两项测定实验检测不同浓度Ag-Ti O2作用后白色念珠菌细胞表面疏水性(CSH)的变化,考察Ag-Ti O2对其黏附能力的影响。结果Ag-Ti O2对白色念珠菌生物被膜具有明显的抑制作用,且呈剂量依赖性,SMIC为400 mg/L;Ag-Ti O2可以干扰白色念珠菌从酵母态向菌丝态的转变,进而抑制三维网状结构的形成,在Ag-Ti O2浓度为300 mg/L时,即可观察到菌丝成分明显减少,被膜结构疏松,极易从附着物上脱落;随着Ag-Ti O2浓度的提高,CSH也随之减低。结论Ag-Ti O2对白色念珠菌生物被膜的形成具有明显的抑制作用,可能是通过干扰菌体菌丝态的形成、降低CSH等方面抑制白色念珠菌的聚集黏附能力。
Objective To study the inhibitory effect of silver-loaded titanium dioxide(Ag-Ti O2)on Candida albicans biofilm,and to explore its mechanism and target.Methods The effect of Ag-Ti O2 on Candida albicans biofilm was detected by XTT method,and the minimum inhibitory concentration(SMIC)of its adhesion was determined.The mycelial growth of Candida albicans was observed under inverted microscope after different concentrations of Ag-Ti O2 were treated,and the effect of Ag-Ti O2 on the transformation from yeast state to mycelial state of Candida albicans was investigated.Finally,the changes of surface hydrophobicity(CSH)of Candida albicans cells treated with Ag-Ti O2 at different concentrations were detected by water-hydrocarbon two-way assay,and the effect of Ag-Ti O2 on its adhesion ability was investigated.Results Ag-Ti O2 inhibited Candida albicans biofilm in a dose-dependent manner,with SMIC of 400 mg/L;Ag-Ti O2 can interfere with the transformation of Candida albicans from yeast to mycelium,and then inhibit the formation of three-dimensional network structure.When Ag-Ti O2 concentration is 300 mg/L,the mycelium can be significantly reduced,the membrane structure is loose and easily falls off from the attachment.With the increase of Ag-Ti O2 concentration,CSH also decreases.Conclusion Ag-Ti O2 could inhibit the formation of biofilm of Candida albicans,possibly by interfering with the formation of mycelium and reducing CSH.
作者
许江燕
王原
邵一为
蒋旭宏
XU Jiang-yan;WANG Yuan;SHAO Yi-wei;JIANG Xu-hong(First Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine,Hangzhou,Zhejiang 310006,China)
出处
《中国卫生检验杂志》
CAS
2020年第14期1668-1670,共3页
Chinese Journal of Health Laboratory Technology
基金
国家发明专利(ZL201110361576.7)
浙江省医药卫生科技计划项目(2018KY551、2018KY552)
浙江省自然科学基金项目(LY14H150004)。
