摘要
背景:慢性病理性疼痛目前形成机制尚不明确,有研究认为脊髓损伤后CCL21可以激活中枢神经中的小胶质细胞,且仅在受损的神经元中表达,促进慢性病理性疼痛的形成。目的:探讨大鼠眶下神经结扎后,前扣带回皮质是否参与慢性病理性疼痛的形成,阻断前扣带回皮质中趋化因子CCL21是否可以减轻疼痛的发生。方法:雄性SD大鼠80只随机分成4组,每组20只。假手术组仅暴露大鼠眶下神经;模型组结扎大鼠左侧眶下神经;CCL21中和抗体组造模术后第7天于大鼠前扣带回皮质给予CCL21中和抗体;PBS组造模术后第7天于大鼠前扣带回皮质给予PBS溶液。假手术组及模型组大鼠分别于术后第3,5,7,14天开展行为学测试,CCL21中和抗体组及PBS组于给药后6 h进行行为学测试。所有大鼠行为学测试后麻醉处死,并取前扣带回皮质组织,采用Western Blot及免疫荧光法测定其中CCL21蛋白含量。结果与结论:①模型组大鼠术后痛阈较假手术组低,且前扣带回皮质中CCL21表达水平明显高于假手术组;②给予CCL21中和抗体之后,可看到CCL21表达有所降低,相对应大鼠痛阈也有相应的提升;③提示大鼠前扣带回皮质可能参与了慢性病理性疼痛的产生,且给予CCL21中和抗体可以改善疼痛。
BACKGROUND:The pathogenesis of chronic pathological pain is yet unknown.Some studies have shown that after spinal cord injury,CCL21 can activate microglia in the central nervous system and is expressed only in damaged neurons,promoting the formation of chronic pathological pain.OBJECTIVE:To investigate whether the anterior cingulate cortex is involved in the formation of chronic pathological pain after inferior orbital nerve ligation in rats,and whether blocking chemokine CCL21 in the anterior cingulate cortex can reduce the chronic neuropathic pain.METHODS:A total of 80 male Sprague-Dawley rats were randomly divided into 4 groups with 20 rats in each group.In the sham group,only the infraorbital nerve of the rats was exposed;in the model group,the left infraorbital nerve was ligated;in the anti-CCL21 group,CCL21 neutralizing antibodies was administered to the anterior cingulate cortex of the rats on the 7th day after surgery;and in the PBS control group,PBS solution was given into the anterior cingulate cortex of rats on the 7th day after surgery.Rats in the sham and model groups were subjected to behavioral tests on the 3rd,5th,7th,and 14th days after surgery,and those in the anti-CCL21 and PBS control groups were subjected to the behavioral test at 6 hours after administration.All rats were sacrificed under anesthesia after behavioral tests.The cortical tissues were taken from the anterior cingulate,and the protein content of CCL21 was determined by western blot and immunofluorescence.RESULTS AND CONCLUSION:The pain threshold of the rats in the model group was lower than that in the sham group,and the expression of CCL21 in the anterior cingulate cortex was significantly higher in the model group than the sham group.After the administration of CCL21 neutralizing antibody,the expression of CCL21 was reduced to some extents,and the rat pain threshold was increased accordingly.These findings reveal that the anterior cingulate cortex of rats may be involved in the production of chronic pathological pain,and the ad
作者
支亦博
章洁
刘健
李伟彦
Zhi Yibo;Zhang Jie;Liu Jian;Li Weiyan(Southern Medical University,Guangzhou 510000,Guangdong Province,China;Jinling Hospital Affiliated to Southern Medical University,Nanjing 210002,Jiangsu Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2021年第2期242-246,共5页
Chinese Journal of Tissue Engineering Research
