摘要
目的观察电针对脊髓损伤后小胶质细胞活化介导炎症的作用,探讨其作用机制。方法36只SD大鼠按随机数字表法分为假手术组、模型组、电针组,每组12只。假手术组仅行椎板切除术,模型组和电针组均采用脊髓打击器制备脊髓损伤模型,自造模后第1天,电针组给予电针干预,其余各组给予同等条件抓取。干预后3 d取材。采用尼氏染色光镜观察神经元尼氏体形态,ELISA法检测大鼠静脉血中IL-1β、IL-18表达水平,激光共聚焦扫描显微镜观察IBA-1/ED-1双阳性细胞数,qPCR检测IL-1、IL-18 mRNA表达情况。结果与模型组比较,电针组IL-1β蛋白[(21.37±0.86)比(31.21±0.86)]、IL-18蛋白[(16.76±0.63)比(23.39±0.97)]、IL-1β mRNA[(3.37±0.86)比(5.21±0.75)]、IL-18 mRNA[(2.76±0.63)比(4.39±0.97)]表达降低(P<0.05),IBA-1/ED-1双阳性细胞数[(7.50±1.18)个比(12.83±1.84)个]减少(P<0.05)。结论电针可促进脊髓损伤后神经元恢复,其作用机制与抑制脊髓损伤后小胶质细胞活化介导的炎症有关。
Objective To observe the effect of electroacupuncture on inflammation mediated by activated microglia after spinal cord injury to explore the mechanism of electroacupuncture inhibiting inflammation and treating spinal cord injury. Methods Thirty-six SD rats were randomly divided into sham group, model group and electroacupuncture group. The sham group was undergone laminectomy while model group and electroacupuncture group with NYU spinal cord impactor for the spinal cord injury model was established. Three days after administration, tissues were gathered for Nissl staining, Elisa, immunofluorescence and qPCR. Results Compared with the model group, the expression of IL-1β (21.37 ± 0.86 vs. 31.21 ± 0.86), IL-18 (16.76 ± 0.63 vs. 23.39 ± 0.97), IL-1β mRNA (3.37 ± 0.86 vs. 5.21 ± 0.75) and IL-18 mRNA (2.76 ± 0.63 vs. 4.39 ± 0.97) in the electroacupuncture group significantly decreased (P<0.05), while the number of IBA-1/ED-1 double positive cells (7.50 ± 1.18 vs. 12.83 ± 1.84) in the electroacupuncture group significantly reduced (P<0.05). Conclusions Electroacupuncture can promote neurons to rehabilitate with a mechanism that electroacupuncture inhibits inflammation mediated by activated microglia after spinal cord injury.
作者
郑鹏
刘宇
Zheng Peng;Liu Yu(The West Coast of Qingdao New District Hospital of Traditional Chinese Medicine, Qingdao 266500, China)
出处
《国际中医中药杂志》
2018年第12期1170-1175,共6页
International Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81503600)
福建省自然科学基金(2015J01684)
福建中医药大学校管课题(2015002-平台).
关键词
脊髓损伤
电针
小神经胶质细胞
炎症
大鼠
Spinal cord injuries
Electroacupuncture
Microglia
Inflammation
Rats
