摘要
[目的]建立HPLC法同时测定泡吴茱萸中吴茱萸碱、吴茱萸次碱和柠檬苦素含量的方法。[方法]采用Agilent 5 TC-C18(2)(250 nm×4.6 nm,5μm)色谱柱,流动相为乙腈-水溶液(53∶47,V/V),等度洗脱,检测波长为215 nm,流动相的流速为1mL/min,色谱柱的柱温为25℃。[结果]在该检测方法下吴茱萸碱、吴茱萸次碱和柠檬苦素分离度良好,3种成分在对应范围内有良好的线性关系;吴茱萸碱、吴茱萸次碱、柠檬苦素的平均回收率分别为98.0%、97.8%、97.3%(n=6)。[结论]该方法简便、准确、分离度高、重复性良好,适用于泡吴茱萸的质量控制。
[Objective]To establish the methods of determining the contents of evodiamine,rutacarpine and limonin in Evodia rutaecarpa by HPLC simultaneously.[Method]Agilent 5 TC-C18(2)(250 nm×4.6 nm,5 μm) chromatographic column was adopted,the mobile phase was acetonitrile-aqueous solution(53∶47,V/V),the same degree of elution,the detection wavelength was 215 nm,the flow rate of mobile phase was 1 mL/min,and the column temperature of chromatographic column was 25 ℃.[Result]The separation of evodiamine,rutaecarpin and limonin was good.The three components were linear in the corresponding range;the average recovery of evodiamine,rutaecarpin and limonin was 98.0%,97.8%,97.3%(n=6),respectively.[Conclusion]The method is simple,accurate and reproducible,and is suitable for the quality control of Evodia rutaecarpa.
作者
周劲松
陈俊禧
罗文英
马恩耀
孔箭
侯皓然
ZHOU Jing-song;CHEN Jun-xi;LUO Wen-ying(Guangzhou Caizhilin Pharmaceutical Co.,Ltd.,Guangzhou,Guangdong 510360;Chinese Medicine Pieces Factory,Guangzhou Medicine Material Corporation,Guangzhou,Guangdong 510360)
出处
《安徽农业科学》
CAS
2020年第16期188-191,共4页
Journal of Anhui Agricultural Sciences
基金
国家重点研发计划中医药现代化研究重点专项(2018YFC1707100)
国家科技重大专项课题(2018ZX09721004-001)
2018年工业转型升级(部门预算)项目(0714-EMTC-02-00576)。
