摘要
以天然水果杨桃为唯一原料,由水热法直接合成了荧光强度高、发光性能良好的杨桃碳量子点(CB-CQDs),用紫外-可见光谱(UV-Vis)、红外光谱(FTIR)、透射电镜(TEM)、荧光光谱(FL)分别表征了CB-CQDs形貌和光学特性。研究发现,在pH 6.0的伯瑞坦-罗宾森(BR)缓冲溶液中,向CB-CQDs溶液中加入银纳米粒子(AgNPs)后,AgNPs与CB-CQDs之间产生荧光共振能量转移(FRET)效应(CB-CQDs和AgNPs分别为荧光供体与受体),使CB-CQDs荧光猝灭,荧光信号"关闭";于该体系中加入西咪替丁(Cimetidine,CMT)后可使猝灭的荧光强度部分恢复,荧光信号重新"打开",据此可实现CMT的定量测定。基于以上原理提出了以CB-CQDs/AgNPs为荧光探针测定CMT的荧光分析新方法,并对反应机理进行了讨论。在优化条件下,CMT浓度在9.0×10^-8~1.0×10^-6 mol/L范围内与CB-CQDs在λex/λem=319 nm/397 nm处的荧光恢复值(ΔIF)呈良好的线性关系,相关系数(r)为0.9986,检出限(3σ/k)为1.9×10^-8 mol/L。将该方法用于样品中CMT的测定,结果满意。
In this paper,a fluorescent probe for the dectection of cimetidine was fabricated based on fluorescence resonance energy transfer effect(FRET)between carambola carbon quantum dots(CB-CQDs)and silver nanoparticles(AgNPs).The CB-CQDs with high fluorescence intensity and good luminescence performance were synthesized by hydrothermal method using natural fruit carambola as the only raw material,which morphology and properties were characterized by ultraviolet-visible spectroscopy(UV-Vis),infrared spectroscopy(FTIR),transmission electron microscopy(TEM)and fluorescence spectrometry(FL),respectively.It was found that,when AgNPs was added into a pH 6.0 Britton-Robinson buffer solution containing CB-CQDs,the fluorescence signal of CB-CQDs atλex/λem=319 nm/397 nm was gradually quenched through the FRET effect,in which CB-CQDs and AgNPs acted as fluorescence donor and fluorescence acceptor,respectively.While in the presence of cimetidine,the fluorescence signal of CB-CQDs was restored and fluorescence signal"turned on"again,forming an"off-on"cimetidine sensor,and the basic mechanism was further discussed.Under the optimized conditions,there was a good linear relationship between fluorescence recovery value(ΔIF)and cimetidine concentration in the range of 9.0×10^-8-1.0×10^-6 mol/L,with a correlation coefficient(r)of 0.9986.The detection limit was calculated to be 1.9×10^-8 mol/L based on the 3σ/k principle.This method was successfully applied to the detection of cimetidine in real samples with satisfactory results.
作者
张洁
张越诚
李承佳
马红燕
李恒
辛程宏
李昊阳
ZHANG Jie;ZHANG Yue-cheng;LI Cheng-jia;MA Hong-yan;LI Heng;XIN Cheng-hong;LI Hao-yang(Yan'an Key Laboratory of Analytical Technology and Detection,College of Chemistry and Chemical Engineering,Yan'an University,Yan'an 716000,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2020年第7期894-899,共6页
Journal of Instrumental Analysis
基金
延安大学科研计划青年项目(YDQ2018-17)
延安大学大学生创新创业训练项目(DC2019-31)。
关键词
杨桃
碳量子点
银纳米粒子
荧光共振能量转移
西咪替丁
carambola
carbon quantum dots
silver nanoparticles
fluorescence resonance energy transfer
cimetidine
