摘要
[目的]研究金花茶组植物花朵及不同花器官中多酚组分的组成和含量,为多酚组分在金花茶植物分类中的应用提供参考。[方法]利用HPLC方法测定金花茶组22种植物全开期花朵的花瓣、雄蕊、萼片3类器官中9种多酚组分含量,并利用聚类分析方法解析其与金花茶组植物分类的关系。[结果]在所测的9种多酚组分中,GCG、ECG、CG在全部样品中均检测到,而GA、GC、EGC、C、EC、EGCG在不等数量的样品中未被检测到。多酚含量最高的组分为EC,其次是EGC、GCG和ECG,剩余5种组分含量很低,前4种组分是花朵多酚总量的主要成分。在3类器官中,多酚总量及EGC、C、EC、EGCG、GCG、ECG 6种组分含量的排序为:萼片>雄蕊>花瓣;CG组分含量的排序为:花瓣>雄蕊>萼片;GA和GC组分含量的排序为:雄蕊>萼片>花瓣。聚类分析发现,雄蕊多酚组分聚类结果与各分类系统的相似率最高,达80.00%~90.00%,萼片多酚分类结果相似率为75.00%~88.89%,花瓣多酚分类结果相似率为58.33%~81.82%。[结论]金花茶花朵中多酚组分含量最高的为EC,主要多酚组分为EC、EGC、GCG和ECG,在3类器官中多酚含量的排序为:萼片>雄蕊>花瓣。雄蕊多酚组分的聚类结果可以作为金花茶组植物系统分类的有效补充。
[Objective] To study the polyphenols in the flowers of yellow Camellia and their relationship with plant classification, and provide reference for the application of polyphenols in the classification of yellow Camellia.[Method] The author used HPLC to determine 9 polyphenolic components in the petals, stamens and sepals of the full flowering flowers of 22 species of yellow Camellia, and the cluster analysis were used to study the relationship between them and the taxonomy of yellow Camellia. [Result] Among the 9 polyphenols tested, GCG, ECG, and CG were detected in all samples, while GA, GC, EGC, C, EC, and EGCG were not detected in some samples. EC was the most abundant, followed by EGC, GCG and ECG. The total polyphenols were significantly positively correlated with these 4 components. The contents of EGC, C, EC, EGCG, GCG, ECG as well as total polyphenols were the highest in sepals, followed by stamens and petals. The content of CG was the highest in petals, followed by stamens and sepals.The contents of GA and GC were the highest in stamens, followed by sepals and petals. The results of cluster analysis showed that the similarity rate of stamen polyphenols with classification systems was the highest, which was80.00%-90.00%. The similarity rates of sepal polyphenols content and petals polyphenols content were 75.00%-88.89% and 58.33%-81.82%, respectively. [Conclusion] The highest polyphenolic content in the flowers of yellow Camellia is EC, and the main polyphenolic constituents are EC, EGC, GCG and ECG. The polyphenolic content is the highest in sepal followed by stamen and lowest in petal. The clustering results of stamen polyphenols can be used as an important supplement to the phylogenetic classification of yellow Camellia.
作者
姜丽娜
李纪元
范正琪
童冉
莫润宏
李志辉
蒋昌杰
JIANG Li-na;LI Ji-yuan;Fan Zheng-qi;TONG Ran;MO Run-hong;LI Zhi-hui;JIANG Chang-jie(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,Zhejiang,China;Nanning Golden Camellia Park,Nanning 530022,Guangxi,China)
出处
《林业科学研究》
CSCD
北大核心
2020年第4期117-126,共10页
Forest Research
基金
国家政府间国际科技创新合作重点专项(2016YFE0126100)
中国林科院中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2017ZF001)。
关键词
金花茶
花
多酚
组分含量
植物分类
yellow Camellia
flower
polyphenols
constituent content
classification
