摘要
【目的】揭示抗逆植物沙拐枣甜菜碱醛脱氢酶(CmBADH)的基因序列。【方法】利用RACE技术克隆CmBADH基因的全长序列,并对其基因序列和蛋白序列进行生物信息学分析。【结果】CmBADH基因的cDNA全长1 863 bp,开放阅读框(ORF)全长1 506 bp,编码501个氨基酸。通过Blastn比对,发现CmBADH与海马齿(Sesuvium portulacastrum)、山谷栎(Quercus lobata)、盐爪爪(Kalidium foliatum)的BADH相似性分别达到80.04%、79.97%、79.55%。其相对分子量为123.46 kDa,等电点(pI)5.01,蛋白序列共有16个磷酸化位点;不含有跨膜结构域;不含有信号肽位点;含有一个甜菜碱醛脱氢酶结构域(PLN02467)。二级结构显示α螺旋占43.31%,无规则卷曲占31.94%,延伸链占17.37%,β转角占7.38%。三级结构模型为椭球形。【结论】沙拐枣CmBADH基因序列的确定,为今后研究沙拐枣CmBADH基因的抗逆功能提供研究基础。
【Objective】To reveal the gene sequence of betaine aldehyde dehydrogenase(CmBADH) in the antistress plant Calligonum mongolicum. 【Methods】RACE technology was used to clone the full-length sequence of CmBADH gene and conduct bioinformatics analysis of its gene and protein sequences.【Results】The cDNA sequence of CmBADH gene was 1 863 bp,and the ORF was 1 506 bp,encoding 501 amino acids.Blastn showed that the BADH similarity between CmBADH and Sesuvium portulacastrum,Quercus lobata,and Kalidium foliatum reached 80.04%,79.97%,and 79.55%,respectively.Its relative molecular weight was 123.46 kDa,isoelectric point(pI) 5.01,and the protein sequence had 16 phosphorylation sites.No transmembrane domain;no signal peptide sites;contains a betaine aldehyde dehydrogenase domain(PLN02467).The secondary structure showed that alpha helix accounts for 43.31%,random coil 31.94%,extended strand 17.37% and beta turn7.38%.The model of tertiary structure is ellipsoid. 【Conclusion】The determination of CmBADH gene sequence provides a basis for future studies on the antistress function of CmBADH gene in Calligonum mongolicum.
作者
房永雨
郝丽芬
聂利珍
丁海君
王力伟
赵小庆
孙杰
史志丹
何江峰
FANG Yongyu;HAO Lifen;NIE Lizhen;DING Haijun;WANG Liwei;ZHAO Xiaoqing;SUN Jie;SHI Zhidan;HE Jiangfeng(Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;Inner Mongolia Key Laboratory of Plant Proteomics,Hohhot 010031,China)
出处
《北方农业学报》
2020年第3期32-38,共7页
Journal of Northern Agriculture
基金
内蒙古自治区农牧业科学院青年创新基金项目(2019QNJJM02)。
