摘要
Calcium ion is a versatile second messenger for diverse cell signaling in response to developmental and environmental cues. The specificity of Ca^2＋-mediated signaling is defined by stimulus-elicited Ca^2＋ signature and down-stream decoding processes. Here, an Aequorin-based luminescence recording system was developed for monitoring Ca^2＋ in response to various stimuli in Arabidopsis. With the simple, highly sensitive, and robust Ca^2＋ recording, this system revealed stimulus-and tissue-specific Ca^2＋ signatures in seedlings. Cellular Ca^2＋ dynamics and relationship to Aequorin-based Ca^2＋ recording were explored using a GFP-based Ca^2＋ indicator, which suggested that a synchronous cellular Ca^2＋ signal is responsible for cold-induced Ca^2＋ response in seedlings, whereas asynchronous Ca^2＋ oscillation contributes to osmotic stress-induced Ca^2＋ increase in seedlings. The optimized recording system would be a powerful tool for the iden-tification and characterization of novel components in Ca^2＋ -mediated stress-signaling pathways,
Calcium ion is a versatile second messenger for diverse cell signaling in response to developmental and environmental cues. The specificity of Ca^2＋-mediated signaling is defined by stimulus-elicited Ca^2＋ signature and down-stream decoding processes. Here, an Aequorin-based luminescence recording system was developed for monitoring Ca^2＋ in response to various stimuli in Arabidopsis. With the simple, highly sensitive, and robust Ca^2＋ recording, this system revealed stimulus-and tissue-specific Ca^2＋ signatures in seedlings. Cellular Ca^2＋ dynamics and relationship to Aequorin-based Ca^2＋ recording were explored using a GFP-based Ca^2＋ indicator, which suggested that a synchronous cellular Ca^2＋ signal is responsible for cold-induced Ca^2＋ response in seedlings, whereas asynchronous Ca^2＋ oscillation contributes to osmotic stress-induced Ca^2＋ increase in seedlings. The optimized recording system would be a powerful tool for the iden-tification and characterization of novel components in Ca^2＋ -mediated stress-signaling pathways,
