摘要
近红外Ⅱb区(near-infrared-Ⅱb,NIR-Ⅱb,1500~1700 nm)荧光因长发射波长,在穿透生物组织时可显著降低光散射及组织自发荧光的干扰,实现更深层影像及更高的空间分辨率。采用硫化铅(PdS)制备了一种NIR-Ⅱb荧光量子点(quantum dots,QDs)。通过控制PbS核的大小调整PbS QDs的发射波长,并采用阳离子交换法生成硫化镉(CdS)壳层,合成核壳结构的PbS/CdS量子点(core/shelled lead sulfide/cadmium sulfide quantum dots,CSQDs)。通过对CSQDs表面进行聚乙二醇(polyethylene glycol,PEG)修饰,提高CSQDs在水溶液中的稳定性。经PEG修饰的CSQDs(PEG-CSQDs)荧光发射峰为~1550 nm,量子效率为7.2%。动物实验经复旦大学药学院实验动物伦理委员会(IACUC)批准。静脉注射PEG-CSQDs 2 h后,可实现对小鼠荷CT26-Luc原位结肠癌模型肿瘤区域清晰的NIR-Ⅱb荧光成像。原位肿瘤与周边正常组织的荧光信号强度比为42.3,转移肿瘤与周边正常组织的荧光信号强度比为22.3。可检测原位瘤的尺寸为3.4 mm×2.5 mm,转移瘤的尺寸为1.2 mm×0.9 mm,并能够准确地指导手术切除肿瘤。本研究所制备的PEG-CSQDs为结肠癌的早期诊断和指导手术切除提供了新方法。
The near-infrared-Ⅱb(NIR-Ⅱb,1500-1700 nm)window fluorescence with long emission wavelength has reduced light scattering and tissue auto-fluorescent background,achieving deep tissue imaging with high spatial resolution.Herein,we prepared an NIR-Ⅱb fluorescent quantum dots(QDs)composed of lead sulfide(PbS).The fluorescence spectrum of PbS QDs were adjusted by controlling the size of the PbS core.Cadmium sulfide(CdS)shell was synthesized by the cation exchange method to form the core/shelled lead sulfide/cadmium sulfide quantum dots(CSQDs).The surface of CSQDs was modified with polyethylene glycol(PEG)to increase their stability in aqueous solution.The resulting PEG-modified CSQDs(PEG-CSQDs)had the emission peak at^1550 nm with quantum yield of 7.2%.The animal procedures were approved by the Institutional Animal Care and Use Committee(IACUC)of Fudan University School of Pharmacy.At 2 h postinjection,PEG-CSQDs clearly delineated the tumor region of mice bearing orthotopic CT26-Luc colon cancer model in the NIR-Ⅱb fluorescence imaging.The fluorescent intensity ratio of primary tumor and adjacent normal tissue was 42.3,and that of metastatic tumor and adjacent normal tissue was 22.3,which allowed to detect the primary tumor of 3.4 mm×2.5 mm in dimension and the metastatic tumor of 1.2 mm×0.9 mm in dimension,and accurately guided the excision of tumors.The PEG-CSQDs prepared in this study provided a new approach for the early diagnosis and guidance of surgical resection of colon cancer.
作者
孙静文
魏国光
钱余义
郑彬彬
高帅
伊庆同
陆伟
SUN Jing-wen;WEI Guo-guang;QIAN Yu-yi;ZHENG Bin-bin;GAO Shuai;YI Qing-tong;LU Wei(Key Laboratory of Smart Drug Delivery,Ministry of Education,State Key Laboratory of Molecular Engineering of Polymers,School of Pharmacy,Fudan University,Shanghai 201203,China;Pelvic Center of Pudong Hospital Affiliated to Fudan University,Shanghai 201399,China)
出处
《药学学报》
CAS
CSCD
北大核心
2020年第7期1699-1706,共8页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(91859110)
“上海市优秀学术带头人”计划(19XD1420200)
上海市浦东新区卫生系统重点学科建设资助项目(PWZxk2017-21)
上海市浦东新区科技发展基金民生科研专项资金资助项目(PKJ2019-Y29)。
