摘要
目的分析比较九味羌活丸剂、颗粒剂和口服液的挥发性成分。方法采用顶空固相微萃取-气相色谱-质谱联用法(HS-SPME-GC-MS)对九味羌活3种剂型的挥发性成分进行分析和比较,用面积归一化法计算各成分的相对百分含量,并进行主成分分析(PCA)。结果从九味羌活丸中鉴定出90种挥发性成分,占总挥发性成分的97.19%;从颗粒剂中鉴定出27种成分,占总挥发性成分的92.92%;从口服液中鉴定出22种成分,占总挥发性成分的87.60%。3种剂型共鉴定出111种挥发性成分,其中共有成分有6种,但共有成分的含量有所差异,丸剂、颗粒剂和口服液含特有成分分别为68、8和13种。并且,3种剂型的PCA综合得分具有较大的差异,其中丸剂的综合得分最高,颗粒剂的综合得分其次,口服液的综合得分最低。结论九味羌活不同剂型的挥发性成分的组成和含量有所差异,并且从挥发性角度看,九味羌活丸剂的综合得分较颗粒剂和口服液高。
Objective To analyze and compare the volatile components of Jiuwei Qianghuo pills,granules and oral liquid.MethodsThe volatile components of Jiuwei Qianghuo pills,granules and oral liquid were analyzed and compared by the head-space solid-phase microextraction and gas chromatography-mass spectrometry(HS-SPME-GC-MS),the relative percentage of each component was calculated using the area normalization method,and the principal component analysis was also performed.ResultsA total of 90 kinds of volatile components were identified from Jiuwei Qianghuo pills,accounting for 97.19% of the total volatile components;27 kinds of volatile components were identified from Jiuwei Qianghuo granules,accounting for 92.92% of the total volatile components;22 kinds of volatile components were identified from Jiuwei Qianghuo oral liquid,accounting for 87.60% of the total volatile components. A total of 111 volatile components were identified in the three preparations,of which 6 were common components,but the contents of the common components were different. The specific components of the pills,granules and oral liquid were 68,8 and13,respectively. Moreover,the comprehensive scores of the three dosage forms in the principal component analysis showed a large difference,with the high,middle and low values for the pills,granules and oral liquid,respectively.ConclusionThe composition and content of volatile components were different in the Jiuwei Qianghuo pill,granule and oral liquid preparations. From the point of view of the volatility,the comprehensive score of Jiuwei Qianghuo pills is higher than that of granules and oral liquid.
作者
韩蔓
江汉美
雷念念
HAN Man;JIANG Han-mei;LEI Nian-nian(Hubei University of Traditional Chinese Medicine,Medicinal Plant Research and Development Center of Hubei Province,Wuhan 430065,China)
出处
《国际药学研究杂志》
CAS
北大核心
2020年第5期395-402,共8页
Journal of International Pharmaceutical Research
基金
湖北省技术创新专项重大项目资助(2016ACA146)。
关键词
顶空固相微萃取
气质联用技术
主成分分析
九味羌活
不同剂型
挥发性成分
head-space solid-phase microextraction(HS-SPME)
gas chromatography-mass spectrometry(GC-MS)
princi-pal component analysis(PCA)
Jiuwei Qianghuo
different preparations
volatile components
