摘要
目的观察电针对颅脑损伤(TBI)后认知障碍(CD)大鼠海马磷酸化认知缺陷突触相关蛋白(SynGAP)的调节作用。方法将80只SD大鼠首先进行水迷宫实验筛选出64只,按照随机数字表法分为空白组10只、造模组54只。造模组行TBI模型制备,又按照水迷宫实验的逃避潜伏期成绩,从用时由多到少依次筛选出34只,随机分为模型组17只、电针组17只。电针组大鼠予电针治疗;空白组、模型组大鼠,每日抓拿1次,常规消毒相应治疗穴位,同样方法固定。比较各组大鼠水迷宫实验结果;光镜下观察各组大鼠海马区脑组织大体形态结构;电镜下观察各组大鼠海马区脑组织超微结构;采用免疫印迹(Western Blot)和免疫组化法检测各组大鼠海马区脑组织中SynGAP含量。结果第1~12次水迷宫实验,模型组、电针组大鼠逃避潜伏期均较空白组延长(P<0.05);第8~12次水迷宫实验,电针组大鼠逃避潜伏期均较模型组短(P<0.05)。模型组大鼠90 s内经过原平台所在区域次数少于空白组(P<0.05);电针组大鼠90 s内经过原平台所在区域次数多于模型组(P<0.05);电针组与空白组大鼠90 s内经过原平台所在区域次数比较差异无统计学意义(P>0.05)。电针组光镜下大体形态结构、电镜下超微结构均有改善。Western Blot检测模型组大鼠海马区脑组织SynGAP相对含量低于空白组(P<0.05),电针组大鼠海马区组织SynGAP相对含量高于模型组(P<0.05)。免疫组化检测模型组大鼠海马CA1、CA3、DG区SynGAP的累积光密度值(IOD)均低于空白组(P<0.05)。电针组大鼠海马CA3、DG区SynGAP的IOD均高于模型组(P<0.05)。结论电针治疗TBI后CD可能参与了神经元突触可塑性的调节,能够改善海马区脑组织超微结构,增加海马CA3、DG区SynGAP含量。
Objective To observe the regulatory effect of electroacupuncture on phosphorylation of synaptic associated proteins(SynGAP)in hippocampus of cognitive deficiency(CD)rats after traumatic brain injury(TBI).Methods 80 SD rats were trained with Morris water maze and divided into blank group(n=10),model group(n=17)and electroacupuncture group(n=17).Except the blank group,the other rats were made CD model after TBI.The rats in the electroacupuncture group were treated by electroacupuncture.The rats in the blank group and the model group were held once a day,and the corresponding treatment points were routinely disinfected and fixed in the same way.The water maze tese results of rats 7n each group were compared.The morphology and ultrastructure of hippocampus were observed under light microscope and electron microscope.The content of phosphorylated SynGAP in hippocampus was detected by Western-blot and immunohistochemistry.Results The latency of water maze in the model group was longer than that in the blank group in the 1st to 12th water maze test(P<0.05).The latency of electroacupuncture group was shorter than that of model group In the 8th to 12th water maze test(P<0.05).The number of times of rats passing through the original platform area in 90 s in the model group was lower than that in the blank group(P<0.05).The number of times of rats passing through the original platform area in 90 s in the electroacupuncture group was higher than that in the model group(P<0.05).There was no significant difference in the times of passing through the original platform area in 90 s between the electroacupuncture group and the blank group(P>0.05).The morphological results of electroacupuncture group were improved under light microscope and electron microscope.The relative percentage of SynGAP in hippocampns of electroacupuncture group was increased detected by Western Blot(P<0.05).The cunulative optical density(IOD)of SynGAP in CA3 and DG areas of hippocampus was significantly increased by Western-blot and immunohistochemistry(P<
作者
郭新荣
李婉露
刘宁
马小卫
GUO Xinrong;LI Wanlu;LIU Ning(School of Acupuncture and Massage,Shaanxi University of Traditional Chinese Medicine,Xianyang,Shanxi 712046)
出处
《河北中医》
2020年第5期731-736,F0002,共7页
Hebei Journal of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(编号:81674088)
中国博士后科学基金资助项目(编号:2014M551038)
2018年陕西省中医药管理局学术流派传承项目-陕西郭氏针灸学术流派传承工作室项目(陕中医药发[2018]40号)
陕西中医药大学学科创新团队项目(编号:2019YL09)。
关键词
脑损伤
并发症
认知障碍
电针
实验动物
Brain injury
Complications
Cognitive impairment
Electroacupuncture
Experimental animals
