摘要
利用血液中含量丰富的糖蛋白—纤维蛋白原,通过纤维蛋白原与还原剂三(2-羧乙基)磷盐酸盐(TCEP)反应,制备了基于纤维蛋白原类淀粉样聚集的纳米薄膜.利用荧光光谱、远紫外-圆二色谱等光学表征手段,探究了纤维蛋白原与TCEP反应的动力学过程及其二级结构的变化;通过透射电子显微镜对薄膜结构进行了表征;用原子力显微镜和光学椭偏仪探讨了反应时间、纤维蛋白原浓度、TCEP溶液pH值以及浓度对薄膜厚度的影响,通过原子力显微镜表征了其在不同溶剂中浸泡的稳定性;最后通过薄膜的血小板吸附实验和对蛋白质的吸附实验,表征了其抗污能力.实验结果表明:通过反应条件的控制,实现了对薄膜厚度的可控,且薄膜在不同的环境中表现出优异的稳定性;薄膜表现出了对血小板、蛋白质、生物体液(如胎牛血清、牛奶等)等非特异性吸附的良好抵抗能力,有望作为新一代生物基抗污材料运用于表/界面改性领域.
The fibrinogen nanofilm was prepared by using the reaction between fibrinogen and the reducing agent tris(2-carboxyethyl) phosphate hydrochloride(TCEP) and resultant amyloid-like aggregation. The kinetics of the reaction between fibrinogen and TCEP and the change of its secondary structure were investigated with optical characterization methods such as fluorescence spectrum and far ultraviolet-circular dichroism. The structure of the thin film was characterized by transmission electron microscopy. The concentration of fibrinogen,the pH value and concentration of TCEP buffer on the thickness of the film were investigated by atomic force microscope and optical ellipsometer. The film stability was characterized by atomic force microscopy. Finally, the antifouling ability of the film was measured by platelet adsorption and protein adsorption. The experimental result shows that by controlling the reaction conditions, the film thickness was controlled, and the film showed excellent stability in different environments. More important, the thin film showed certain resistance to platelet adsorption and model biofluid mixtures(e.g., fetal bovine serum, milk). Therefore, the fibrinogen amyloid-like aggregation film reported in this article is expected to be used as a new type of bio-based materials in anti-thrombosis, vascula stent coating, anti-fouling coating and other biomedical engineering fields. The idea of phase-transited protein with strong non-specific adsorption through amyloid aggregation into anti-fouling coatings that resist non-specific adsorption of other molecules can inspire us to explore more anti-fouling systems based on functional protein aggregation mechanism.
作者
杨庆敏
刘永春
陈立新
杨鹏
Qing-min Yang;Yong-chun Liu;Li-xin Chen;Peng Yang(Key Laboratory of Applied Surface and Colloid Chemistry,Ministry of Education,School of Chemistry and Chemical Engineering,Shaanxi Normal University,Xi’an 710119;School of Chemistry and Chemical Engineering,Northwestern Polytechnical University,Xi’an 710072)
出处
《高分子学报》
SCIE
CAS
CSCD
北大核心
2020年第8期890-900,共11页
Acta Polymerica Sinica
基金
国家自然科学基金委面上项目(基金号51673112)
应用表面与胶体化学111引智基地(项目号B14041)
陕西师范大学中央高校基本科研业务费(项目号GK201801003,2017CBY004)。
