摘要
【目的】探讨腹腔注射四氢姜黄素(THC)对过敏性哮喘小鼠呼吸道症状和炎症反应的治疗效果。【方法】BALB/c小鼠随机分为4组,包括:正常对照组、卵清蛋白(OVA)诱导动物模型组、阳性对照组和THC治疗组。其中,阳性对照组和THC治疗组小鼠于实验第21~25天,分别经腹腔注射2.5 mg/kg地塞米松(DEX)或20 mg/kg THC。记录小鼠激发前后体质量变化和挠鼻情况,实验结束后处死小鼠,采集肺组织和肺泡灌洗液(BALF)评估组织病理改变、检测T细胞亚型及相关细胞因子水平。【结果】哮喘小鼠经THC腹腔注射治疗后挠鼻频率降低(P<0.05),两组均数之差为5.2,其95%置信区间(CI)为0.66~9.74;肺组织病理评分减少(P<0.05),两组均数之差为1.6,其95%CI为0.32~2.88;杯状细胞增生引起的粘液分泌水平减轻(P<0.0001),两组均数之差为9.56,其95%CI为5.05~14.07。此外,与OVA组相比,THC治疗组小鼠肺组织Th2、Th17细胞比例减少(P<0.01,两组均数之差分别为1.492和2.15,其95%CI分别为0.50~2.49和0.72~3.58),BALF中白细胞介素(IL)IL-4、IL-5、IL-13和IL-17A的水平显著降低(P<0.05),两组均数之差分别为5.45、4.13、5.17和2.44,其95%CI分别为1.95~8.94、1.08~7.19、0.80~9.54和-0.30~5.17。进一步比较发现THC与DEX治疗组间肺组织病理变化、Th17细胞以及IL-13和IL-17A因子水平差异无统计学意义(P>0.05)。【结论】腹腔注射THC可以在短时间内有效缓解哮喘小鼠呼吸道症状和抑制炎症反应,且安全性较高,因此可能具有哮喘发作时替代性治疗手段的潜在价值。
【Objective】To study the therapeutic effects of tetrahydrocurcumin(THC)via intraperitoneal(i.p.)injection on the respiratory symptoms and inflammatory responses in asthmatic mice.【Methods】BALB/c mice were randomly divided into 4 groups,including normal control group,ovalbumin(OVA)-induced model group,positive group and THC administration group.The latter two groups were treated with 2.5 mg/kg of dexamethasone(DEX)or 20 mg/kg of THC via i.p.injection from Day 21 to Day25.The weight changes and nasal symptoms were recorded before and after OVA chal⁃lenge.The mice were sacrificed at the end of the experiment and the lung tissue and broncho-alveolar lavage fluid(BALF)were collected for assessment of histopathological alterations,Th cell subsets and related cytokine level.【Results】After THC treatment via i.p.injection,the asthmatic mice’s rubbing frequencies were reduced(P<0.05)with a difference of 5.2 between the two means(95%CI=0.66 to 9.74),lungs’pathological scores were reduced(P<0.05)with a difference between the two means being 1.6(95%CI=0.32 to 2.88),mucus production induced by hyperplasia of goblet cells was alleviated(P<0.0001)with a difference between the two means being 9.56(95%CI=5.05 to 14.07).Besides,compared with OVA group,the percentages of Th2 and Th17 cells were reduced(P<0.01)with a difference between the two means being 1.49 and 2.15(95%CI=0.50 to 2.49 and 0.72 to 3.58),respectively.The levels of interleukin(IL)-4,IL-5,IL-13 and IL-17A in BALF were decreased(P<0.05)with a difference between the two means being 5.45,4.13,5.17 and 2.44(95%CI=1.95 to 8.94,1.08 to 7.19,0.80 to 9.54 and-0.30 to 5.17),respectively.The further comparison between THC and DEX groups showed no significant difference(P>0.05)in the lung pathological change,Th17 cells,IL-13,and IL-17A levels.【Conclusions】It is concluded that i.p.injection of THC could effectively alleviate the respiratory symptoms and inhibit inflammatory reaction in asthmatic mice in short time with high safety.Therefore,i.p.injection of T
作者
武音帆
李琴
邹进超
毛钰蘅
郭兴悦
陈彦球
杨燕
WU Yin-fan;LI Qin;ZOU Jin-chao;MAO Yu-heng;GUO Xing-yue;CHEN Yanqiu;YANG Yan(School of Public Health,Sun Yat-sen University,Guangzhou 510080,China;Guangdong Provincial Key Laboratory of Food,Nutrition and Health,School of Public Health,Sun Yat-sen University(Guangzhou Campus),Guangzhou 510080,China;Guangdong Engineering Technology Research Center of Nutrition Translation,Guangzhou 510080,China;School of Public Health(Shenzhen),Sun Yat-sen University,Shenzhen 518000,China;Department of Otolaryngology,Guangzhou Women and Children Medical Centre,Guangzhou 510623,China)
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2020年第4期525-533,共9页
Journal of Sun Yat-Sen University:Medical Sciences
基金
深圳市科技创新计划(201803073000433)
中山大学实验室开放基金(20180278)。
关键词
四氢姜黄素
过敏性哮喘
治疗
腹腔注射
炎症反应
tetrahydrocurcumin
allergic asthma
therapy
intraperitoneal injection
inflammatory response
