摘要
该研究以南瓜果实为原料,利用超声辅助乙醇浸提法对南瓜果实黄酮类化合物的提取工艺条件进行优化,并对其抗氧化性进行研究。在单因子试验基础上,采用Design-Expert 8.0.6进行试验设计,采用曲面响应法对最佳黄酮提取条件进行优化,构建预测模型的二次多项式回归方程。结果表明,南瓜果实黄酮类化合物提取最佳条件为:料液比1∶20、乙醇浓度70%、超声时间30 min、提取温度60℃、预浸泡时间6 h、粉粹粒径120目。南瓜果实黄酮提取物对DPPH·、ABTS+·、·OH具有不同程度的清除作用,3种不同自由基的清除率与南瓜黄酮提取物浓度之间存在一定量效关系。当南瓜果实黄酮提取物浓度为0.084,0.072,0.2 mg/mL时,分别对DPPH·、ABTS+·、·OH的清除效果最好,清除率依次为88.55%、89.34%、98.34%。
In this study,pumpkin fruits are used as the raw materials to optimize the extraction technology conditions for flavonoids from pumpkin fruit by ultrasonic-assisted ethanol extraction method,and the antioxidant activity of flavonoids is studied.Based on the single factor test,Design-Expert 8.0.6 is used to design the experiment and surface response method is used to optimize the extraction conditions for flavonoids.The quadratic polynomial regression equation of the prediction model is constructed.The results show that the optimum conditions for extracting flavonoids from pumpkin fruits are as follows:the ratio of solid to liquid is 1∶20,the concentration of ethanol is 70%,the ultrasonic time is 30 min,the extraction temperature is 60,the pre-soaking time is 6 h,and the crushing particle size is 120 meshes.The flavonoids extracts from pumpkin fruits have different scavenging effects on DPPH·,ABTS+·and·OH and there's a relationship between the scavenging rates of three different free radicals and the concentration of flavonoids extracts from pumpkin fruits.When the concentration of flavonoids extracts from pumpkin fruits is 0.084,0.072,0.2 mg/mL,the scavenging effect on DPPH·,ABTS+·and·OH is the best,and the scavenging rate is 88.55%,89.34%and 98.34%respectively.
作者
李娜
燕平梅
乔宏萍
张志军
李晓君
贺东亮
LI Na;YAN Ping-mei;QIAO Hong-ping;ZHANG Zhi-jun;LI Xiao-jun;HE Dong-liang(Taiyuan Normal University,Jinzhong 030619,China;North University of China,Taiyuan 030051,China;Taiyuan Institute of Technology,Taiyuan 030008,China)
出处
《中国调味品》
CAS
北大核心
2020年第8期24-30,共7页
China Condiment
基金
山西省高等学校科技创新项目(2019L0819)。
关键词
南瓜果实
黄酮类化合物
提取工艺
响应面法
抗氧化性
pumpkin fruits
flavonoids
extraction technology
response surface method
antioxidant activity
