摘要
目的探讨交叉引物恒温扩增(TB-CPA)法、液体培养法及传统聚合酶链反应(PCR)法在结核性胸膜炎临床诊断中的应用价值。方法选取该院2017年6月至2019年6月接收的119例胸腔积液患者为研究对象,其中经生化、超声波检查及胸膜活检等确诊为结核性胸膜炎患者101例,分别采用TB-CPA法、液体培养法及传统PCR法进行诊断,以受试者工作特征曲线(ROC曲线)为依据,对3种方法结核性胸膜炎阳性检出率、诊断准确性、灵敏度、特异度等情况进行分析。结果TB-CPA法的结核性胸膜炎阳性检出率较液体培养法、传统PCR法均更高,差异有统计学意义(P<0.05)。TB-CPA法诊断准确性、灵敏度、特异度较液体培养法及传统PCR法均更高,差异有统计学意义(P<0.05)。TB-CPA法、液体培养法、传统PCR法诊断ROC曲线下面积分别为0.837、0.715、0.809,差异有统计学意义(P<0.05)。结论TB-CPA法诊断结核性胸膜炎更具优势,可准确、快速检出疾病。
Objective To explore the value of cross primer isothermal amplification(TB-CPA),liquid culture and traditional PCR in the clinical diagnosis of tuberculous pleurisy.Methods Totally 119 patients with pleural effusion received by the hospital from June 2017 to June 2019 were selected as the research objects,among which 101 patients with tuberculous pleurisy were confirmed by biochemical examination,ultrasonic examination and pleural biopsy.TB-CPA,liquid culture and traditional PCR method were used for diagnosis respectively.Based on receiver operating characteristic curve(ROC curve),the positive detection rate,diagnostic accuracy,sensitivity and specificity of the three methods were analyzed.Results The positive detection rate of tuberculous pleurisy by TB-CPA method was higher than that by liquid culture method and traditional PCR method(P<0.05).The diagnostic accuracy,sensitivity and specificity of TB-CPA method were higher than those of liquid culture method and traditional PCR method(P<0.05).The area under the ROC curve of TB-CPA method,liquid culture method and traditional PCR method were 0.837,0.715 and 0.809 respectively,the difference was statistically significant(P<0.05).Conclusion TB-CPA method has more advantages in the diagnosis of tuberculous pleurisy,which can accurately and quickly detect the disease.
作者
王超
徐东芳
彭远远
王庆
WANG Chao;XU Dongfang;PENG Yuanyuan;WANG Qing(Department of Clinical Laboratory,Anhui Chest Hospital,Hefei,Anhui 230022,China)
出处
《检验医学与临床》
CAS
2020年第15期2119-2121,共3页
Laboratory Medicine and Clinic
关键词
结核性胸膜炎
交叉引物恒温扩增
液体培养法
聚合酶链反应
结核分枝杆菌
tuberculous pleurisy
cross primer isothermal amplification
liquid culture
polymerase chain reaction
Mycobacterium tuberculosis
