摘要
目的探究抗酸染色联合金胺O荧光染色诊断肺结核的临床价值。方法选取2015年1月—2017年12月深圳市盐田区人民医院收治并确诊的肺结核患者200例作为肺结核组,选取同期于深圳市盐田区人民医院进行病理组织学诊断的非肺结核患者50例作为对照组;两组患者均行组织抗酸染色、痰液抗酸染色法及金胺O荧光染色;比较三种检测方法分别及联合诊断肺结核的诊断效能。结果两组抗酸染色及金胺O荧光染色对肺结核诊断的阳性率均不高,但对非结核类疾病诊断的阴性率均较高,分别为100.00%、100.00%及94.00%;与金胺O荧光染色相比,两组抗酸染色的灵敏度、特异性、准确性及阳性预测率均有显著增加(P<0.05);三种方法联合检测对肺结核患者诊断的阳性率为88.00%(176/200),阴性率为12.00%(24/200);对非结核患者诊断的阳性率为6.00%(3/50),阴性率为94.00%(47/50);灵敏度、特异性、准确性、阳性预测率及阴性预测率分别为88.00%、94.00%、98.32%、66.20%及80.00%。与单独检测相比,灵敏度、准确度及阳性预测率得到显著提升。结论抗酸染色联合金胺O荧光染色能够显著提升肺结核患者诊断的灵敏度、准确度及阳性预测率,具有显著的临床意义。
Objective To explore the clinical value of acid-fast staining combined with gold amine O fluorescence staining in the diagnosis of pulmonary tuberculosis.Methods A total of 200 patients with pulmonary tuberculosis admitted to the hospital from January,2015 to December,2017 were enrolled as the tuberculosis group.50 patients with non-pulmonary tuberculosis who underwent histopathological diagnosis in the hospital were selected as the control group.The two groups underwent tissue acid-fast staining,sputum acid-fast staining and auramine O fluorescence staining;the diagnostic efficacy of the three methods and combined diagnosis of tuberculosis were compared.Results The positive rates of anti-acid staining and auramine O fluorescence staining for pulmonary tuberculosis were not high,but the negative rates for non-binding diseases were higher,which were 100.00%,100.00%and 94.00%,respectively.Compared with the fluorescence staining of auramine O,the sensitivity,specificity,accuracy and positive predictive rate of the two groups of acid-fast staining increased significantly(P<0.05).The positive rate of combined detection of three methods for pulmonary tuberculosis patients was 88.00%(176/200),the negative rate was 12.00%(24/200).And the positive rate and negative rate for non-tuberculosis patients was 6.00%(3/50)and 94.00%(47/50).The sensitivity,specificity,accuracy,positive predictive rate and negative predictive rate of the combined detection of three methods for pulmonary tuberculosis patients were 88.00%,94.00%,98.32%,66.20%and 80.00%,respectively.Sensitivity,accuracy and positive predictive rate improved significantly compared with individual tests.Conclusion Anti-acid staining combined with auramine O fluorescence staining can significantly improve the sensitivity,accuracy and positive predictive rate of pulmonary tuberculosis patients,and has significant clinical significance.
作者
廖子龙
徐加誉
陈宝玉
李勇
LIAO Zi-long;XU Jia-yu;CHEN Bao-yu(Department of Pathology,Shenzhen Yantian People’s Hospital,Shenzhen,Guangdong,518000,China)
出处
《黑龙江医学》
2019年第4期394-395,398,共3页
Heilongjiang Medical Journal
基金
深圳市盐田区科技计划项目(20160101)
关键词
抗酸染色
金胺O荧光染色
肺结核
临床诊断效能
Acid-fast staining
Auramine O fluorescence staining
Tuberculosis
Clinical diagnostic efficacy
