摘要
运用正交回归试验设计,研究了肥料和密度互作下蒙啤麦5号灌浆期叶片抗氧化酶活性的变化。结果表明:在氮肥64.5~97.5kg/hm^2,磷肥79.05~135.00kg/hm^2,钾肥26.55~60.00kg/hm^2,种植密度458.00~528.00万株/hm^2范围内,增加氮、磷、钾施用量和种植密度均可提高灌浆期蒙啤麦5号叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,超过此范围,则其活性下降。从4个因素的偏回归系数看,其对SOD、POD、CAT活性的影响表现为磷肥>钾肥>氮肥>种植密度,说明灌浆期增施磷肥对蒙啤麦5号抗氧化酶活性的促进作用最明显。在该试验条件下,蒙啤麦5号灌浆期SOD活性最高理论值为241.65U/g,POD活性最高理论值为1077.92U/g·min,CAT活性最高理论值为44.83U/g·min。达到叶片抗氧化酶活性理论最高值的最佳施肥和密度为氮肥(N)102.10~114.30kg/hm^2,磷肥(P2O5)95.27~129.98kg/hm^2,钾肥(K2O)19.52~86.53kg/hm^2,密度447.50~621.93万株/hm^2。
The changes of chlorophyll fluorescence parameters of Mengpimai5 under the interaction of fertilizer and density was studied by orthogonal regression experiment.The results showed that increasing nitrogen,potassium fertilizer and planting density could increase the superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)activities of Mengpimai5 in heading date within 64.5-97.5 kg/hm^2 nitrogen fertilizer,79.05-135.00 kg/hm^2 phosphorus fertilizer,26.55-60.00 kg/hm^2 potassium fertilizer and4.58-5.28 million plants/hm^2 density,but decreased beyond these range.The results of regression analysis of interaction effects of fertilizer and density revealed that the factors affecting the activities of SOD,POD and CAT were phosphate fertilizer,potash fertilizer,nitrogen fertilizer and planting density,indicating that increasing phosphate fertilizer could increase the antioxidant enzyme activities of Mengpimai5 during grain filling.Under the conditions of this test,theoretical maximum values of SOD,POD and CAT activities of Mengpimai5 were 241.65 U/g,1077.92 U/(g·min)and 44.83 U/(g·min),respectively.The optimum fertilization and density with the highest theoretical value of antioxidant enzyme activity in leaves were ranging of 102.10-114.30 kg/hm^2 of nitrogen fertilizer(N),95.27-129.98 kg/hm^2 of phosphate fertilizer(P2 O5),19.52-86.53 kg/hm^2 of potash fertilizer(K2 O),447.50-621.93 million plants/hm^2 of density.
作者
刘志萍
王君
巴图
马宇
尚学燕
姜晓平
沈秋云
徐寿军
LIU Zhiping;WANG Jun;BA Tu;MA Yu;SHANG Xueyan;JIANG Xiaoping;SHEN Qiuyun;XU Shoujun(Institute of Agronomy,Inner Mongolia Academy of Agricultural Sciences,Huhhot Inner Mongolia 010031,China;Wuhai City Agricultural Mechanization Service and Management Center,Wuhai Inner Mongolia 016000,China;Agriculture and Animal Husbandry Technology Promotion Center of Dengkou County,Bayannaoer City,Bayangole Inner Mongolia 015200,China;College of Agronomy,Inner Mongolia University for Nationalities,Tongliao Inner Mongolia 028043,China)
出处
《延边大学农学学报》
2020年第2期58-64,共7页
Agricultural Science Journal of Yanbian University
基金
国家自然科学基金项目(31760357,31360307)
内蒙古自治区科技计划项目(2019GG342)
内蒙古自治区草原英才工程项目。
关键词
大麦
肥料
种植密度
抗氧化酶
Barley
Fertilizer
Planting density
Antioxidant enzyme activity
