摘要
目的观察吸附排尘中草药制剂(以下简称"XFPC")对矽肺大鼠肺组织炎症及纤维化的干预作用。方法选择无特定病原体级雄性SD大鼠144只,随机分为空白对照组、矽肺模型组、给药对照组和XFPC低、中、高剂量组,每组24只。除空白对照组外,其余5组大鼠采用单次吸入式气管滴注法予质量浓度为50 g/L二氧化硅混悬液制备矽肺模型。于造模第7天开始给药干预,其中给药对照组大鼠予剂量为5 mg/kg体质量汉防己甲素灌胃,XFPC低、中、高剂量组大鼠分别予质量浓度为43、86、192 g/L的XFPC雾化吸入,每日1次,每次20 min,每周给药5 d,连续4周。给药结束后,观察各组大鼠肺组织病理学改变,采用形态学检测和酶联免疫吸附测定方法检测大鼠支气管肺泡灌洗液(BALF)中细胞数量、分类及丙二醛(MDA)、干扰素-γ(IFN-γ)水平。结果造模后7 d,与空白对照组比较,给药对照组和XFPC高剂量组大鼠体质量均降低(P<0.05);造模后35 d,其余5组大鼠体质量均低于同时间点空白对照组(P<0.05)。给药对照组和XFPC低剂量组大鼠肺组织肉眼和光镜下(炎细胞浸润、纤维化程度及矽结节大小)肺组织病理学改变均较矽肺模型组改善。与矽肺模型组比较,给药对照组和XFPC低剂量组大鼠肺脏脏器系数和BALF中中性粒细胞比例以及MDA、IFN-γ水平均降低(P<0.05),BALF中巨噬细胞比例均升高(P<0.05)。XFPC中、高剂量组大鼠肺脏脏器系数和BALF中有关指标分别与矽肺模型组比较,差异均无统计学意义(P>0.05)。结论低剂量XFPC对矽肺大鼠肺纤维化和炎症具有一定改善作用,其机制可能与降低MDA和IFN-γ的水平有关。
Objective To investigate the interventional effect of the Chinese herbal preparation Xi Fu Pai Chen(XFPC)on pulmonary inflammation and fibrosis in rats with silicosis.Methods A total of 144 adult specific pathogen free male SD rats were randomly divided into 6 groups:blank control group,silicosis model group,drug administration control group and groups of low-dose,medium-dose and high-dose XFPC,with 24 rats in each group.Lung silicosis model was established by single inhalation tracheal instillation method,which was treated with 50.0 g/L silica suspension,in groups except in the blank control group.On the 7th day of modeling,the rats in the drug administration control group were orally given tetrandrine(5 mg/kg body weight),while those in the low-,medium-and high-dose groups were given 43,86 and 192 g/L of XFPC by atomization inhalation once a day for 20 minutes,5 days a week for 4 weeks.At the end of drug administration,the histopathological changes of the lung were observed.The number and classification of cells in bronchoalveolar lavage fluid(BALF)were examined,and the levels of malondialdehyde(MDA)and interferon-gamma(IFN-γ)in BALF were measured by enzyme-linked immunosorbent assay.Results On the 7th day after modeling,the body weight in the drug administration control group and XFPC high-dose group decreased compared with the blank control group(P<0.05).On the 35th day after modeling,the body weights of rats in the other 5 groups were lower than that in the blank control group(P<0.05).The pathological changes of lung tissue(infiltration of inflammatory cells,fibrosis and size of silicon nodule)in drug administration control group and XFPC low-dose group were better than those in silicosis model group by naked eyes and under light microscope.The lung coefficient,the proportion of neutrophils and the level of MDA and IFN-γin BALF of the drug administration control group and XFPC low-dose group decreased(P<0.05),and the proportion of macrophages in BALF increased(P<0.05)compared with the silicosis model group
作者
赵宏艳
刘红
潘静华
王少君
李艳
卓来宝
武佳佳
轩敏晖
付晓丽
燕贞
张德旺
蒋盛军
ZHAO Hongyan;LIU Hong;PAN Jinghua;WANG Shaojun;LI Yan;ZHUO Laibao;WU Jiajia;XUAN Minhui;FU Xiaoli;YAN Zhen;ZHANG Dewang;JIANG Shengjun(Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China;不详)
出处
《中国职业医学》
CAS
北大核心
2020年第2期190-195,共6页
China Occupational Medicine
