摘要
目的探讨岩盐气溶胶对大鼠矽肺发生发展的预防作用。方法将无特定病原体级成年雄性SD大鼠随机分为正常对照组、岩盐对照组、矽肺模型组和岩盐干预组,每组18只。矽肺模型组和岩盐干预组大鼠均以动式吸入染尘法予质量浓度为2000.0 mg/m^3游离二氧化硅粉尘,每日染尘3 h;岩盐对照组和岩盐干预组大鼠均予吸入质量浓度为20.0 mg/m^3的岩盐气溶胶,每日30 min。正常对照组大鼠不予染尘和岩盐干预处理。分别于染尘14、28、56 d,每组随机抽取6只大鼠,观察其肺组织病理学改变情况,检测大鼠支气管肺泡灌洗液(BALF)中总细胞计数,并采用酶联免疫吸附实验检测大鼠BALF中转化生长因子-β(TGF-β)和肺组织中表面活性物质D(SP-D)、超氧化物歧化酶(SOD)变化。结果苏木素-伊红和Masson染色结果显示,岩盐干预组大鼠肺组织炎性改变和肺间质纤维化进展均较矽肺模型组缓慢。在染尘14、28、56 d时间点,与正常对照组和岩盐对照组比较,矽肺模型组和岩盐干预组大鼠BALF中总细胞计数和肺组织中SP-D水平均升高(P<0.05),肺组织中SOD活力均下降(P<0.05),且矽肺模型组大鼠BALF中TGF-β水平均升高(P<0.05);而与矽肺模型组比较,岩盐干预组大鼠BALF中总细胞计数、TGF-β水平和肺组织中SP-D水平均下降(P<0.05),肺组织中SOD活力均升高(P<0.05)。结论岩盐气溶胶干预可能通过改善游离二氧化硅所致大鼠肺部炎症反应,调节氧化应激水平,降低肺间质纤维化程度,进而延缓矽肺的发生发展。
Objective To investigate the preventive effect of rock salt aerosol on the development of silicosis in rats.Methods The specific pathogen free adult male SD rats were randomly divided into normal control group,rock salt control group,silicosis model group and rock salt intervention group,18 rats in each group.Rats in the silicosis model group and the salt rock intervention group were treated with silica dust at the concentration of 2000.0 mg/m3 by dynamic dusting method for 3 hours daily.Rats in the rock salt control group and the rock salt intervention group inhaled the rock salt aerosols with the mass concentration of 20.0 mg/m3 for 30 minutes daily.The normal control group was not treated with the dust or rock salt aerosol.At the time points of 14,28 and 56 days after exposure to dust or rock salt aerosol,6 rats were randomly selected from each group and samples were collected.The pathological change of lung was observed,the total cell count in the bronchoalveolar lavage fluid(BALF)was performed,the enzyme-linked immunosorbent assay was used to detect the change of transforming growth factor-β(TGF-β)in BALF,surfactant D(SP-D)and superoxide dismutase(SOD)in lung tissue.Results The results of hematoxylin-eosin and Masson staining showed that the inflammatory changes of lung tissue and the pulmonary interstitial fibrosis in the rock salt intervention group were less severer than that in the silicosis model group.At 14,28,and 56 days after dust exposure,the total cell counts in BALF and SP-D levels in lung tissue of rats in silicosis model group and rock salt intervention group were higher(P<0.05),the SOD activities in lung tissue were lower(P<0.05),as well as the TGF-βlevels in BALF in silicosis model group were higher(P<0.05),compared with the normal control group and rock salt control group.The total cell counts and TGF-βlevels in BALF,and SP-D levels in lung tissue of rock salt intervention group were lower(P<0.05),the SOD activities in lung tissue were higher(P<0.05),compared with the silicosis model grou
作者
王爽
赵晓坤
朱嘉莉
徐秋敏
李欣颖
张劲松
郝小惠
郭灵丽
刘和亮
WANG Shuang;ZHAO Xiaokun;ZHU Jiali;XU Qiumin;LI Xinying;ZHANG Jinsong;HAO Xiaohui;GUO Lingli;LIU Heliang(School of Public Health,North China University of Science and Technology,Tangshan,Hebei 063210,China;不详)
出处
《中国职业医学》
CAS
北大核心
2020年第2期147-153,共7页
China Occupational Medicine
基金
中国煤矿尘肺病防治基金会科研项目(201601-3)。
关键词
矽肺
岩盐
气溶胶
预防
炎性反应
纤维化
大鼠
Silicosis
Rock salt
Aerosol
Prevention
Inflammatory response
Fibrosis
Rat