摘要
目的通过对窒息法大鼠心脏骤停-心肺复苏(CA-CPR)模型的观察,探讨影响大鼠CA-CPR模型造模成功率与预后的相关因素。方法将50只SD大鼠根据其体重分为Ⅰ组(10只,250~300 g)、Ⅱ组(30只,300~350 g)、Ⅲ组(10只,350~400 g),通过窒息法建立CA-CPR模型,记录并比较三组大鼠基线资料、大鼠自主循环恢复(ROSC)成功率、心肺复苏(CPR)时间、ROSC后的血流动力学情况等。结果Ⅰ组、Ⅱ组、Ⅲ组ROSC成功率分别为90.00%(9/10)、60.00%(18/30)、60.00%(6/10),无统计学差异(P>0.05)。Ⅰ组的CPR时间(365.16±76.66)s,与Ⅱ组(417.19±92.00)s、Ⅲ组(60.80±115.40)s比较,均存在统计学差异(P<0.05)。ROSC后的血流动力学比较,Ⅰ组大鼠在ROSC后第30 min的心率(HR)比Ⅱ组大鼠((306.22±41.99)比(264.47±33.28)次/分),存在统计学差异(P<0.05),余时间段比较无统计学差异(均P>0.05)。多因素二分类Logistic回归分析,将体重、肛温、HR、平均动脉压、窒息致心脏骤停(ACA)时间、CA持续时间,分别纳入是否ROSC(χ2=25.115,P<0.001)、是否存活72 h(χ2=14.191,P=0.001)的获得的回归方程具有统计学意义。其中ROSC与ACA时间OR为1.087,95%置信区间(95%CI):1.031-1.146;存活72 h与体重OR=0.953,95%CI:0.915-0.992、HR OR=0.957,95%CI:0.921-0.994。结论在标准造模条件下,体重较轻的成年鼠,具有较高的可能实现ROSC并成功建模;大鼠的基线心率、体重与大鼠的生存结局成负相关,ACA时间与良好的结局呈正相关。
Objective To explore factors affecting the success of and prognosis for cardiac arrest-cardiopulmonary resuscitation(CA-CPR)in a rat model induced by asphyxia.Methods The CA-CPR model was established in 50 SD rats,which were divided into three groups according to weight:groupⅠ(n=10,250~300 g),groupⅡ(n=30,300~350 g),and groupⅢ(n=10,350~400 g).Baseline data,the rate of return of spontaneous circulation(ROSC),and the time needed for cardiopulmonary resuscitation(CPR)were recorded,and the hemodynamics after ROSC were assessed.Results The ROSC rates for groupⅠ,groupⅡ,and groupⅢwere 90.00%(9/10),60.00%(18/30),and 60.00%(6/10),respectively,and were not significantly different(P>0.05).Compared with groupⅡ(417.19±92.00)s and groupⅢ(60.80±115.40)s,groupⅠexhibited a significantly shorter CPR time(365.16±76.66;P<0.05).The heart rate(HR)of rats in groupⅠ(306.22±41.99 beats/min)30 minutes after ROSC was significantly different to that of rats in groupⅡ(264.47±33.28 beats/min;P<0.05),and there were no significant differences between the other groups(all P>0.05).A binary logistic regression model was used to evaluate the predictive index for ROSC(χ2=25.115,P<0.001)and 72-hour survival(χ2=14.191,P=0.001).Six items were included:body weight,anal temperature,HR,mean arterial pressure,asphyxia to cardiac arrest(ACA)time,and CA duration.ROSC was positively correlated with ACA time(OR=1.087,95%confidence interval(95%CI):1.031-1.146);and 72-hour survival was negatively related to body weight(OR=0.953,95%CI:0.915-0.992)and HR(OR=0.957,95%CI:0.921-0.994).Conclusions Our findings show that rats with lower weight,lower baseline HR,and longer ACA time were more likely to have a good outcome.
作者
曾瑞峰
钟悦嘉
赖成志
赖芳
刘相圻
段云彪
李尊江
吴炎华
李俊
丁邦晗
ZENG Ruifeng;ZHONG Yuejia;LAI Chengzhi;LAI Fang;LIU Xiangqi;DUAN Yunbiao;LI Zunjiang;WU Yanhua;LI Jun;DING Banghan(the Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangdong Provincial Hospital of Chinese Medicine,Guangdong Provincial Key Laboratory of Research on Emergency in TCM,Guangzhou 510120,China;Guangzhou University of Chinese Medicine,Guangzhou 510006;Shenzhen Hospital of Guangzhou University of Chinese Medicine,Shenzhen 518034;Wuyi Traditional Chinese Medicine Hospital of Jiangmen,Jiangmen 529000)
出处
《中国比较医学杂志》
CAS
北大核心
2020年第7期65-71,共7页
Chinese Journal of Comparative Medicine
基金
2017年广东省科技厅项目(2017ZC0164)
广东省中医急症研究重点实验室(2017B030314176)
2018年广州市科技计划项目(201803010030)。
关键词
心脏骤停
心肺复苏
动物模型
窒息
cardiac arrest
cardiopulmonary resuscitation
animal model
asphyxia
