摘要
【目的】探索杂交兰EST-SSR标记在国兰中的通用性,为国兰寻找新的分子标记,并利用这些新标记分析国兰的种间遗传多样性,以期为国兰的品种鉴定和种质资源的合理利用奠定基础。【方法】以建兰、墨兰、春兰等国兰44个品种为试验材料,对240对杂交兰EST-SSR标记引物的通用性进行研究。在此基础上,通过筛选的引物对国兰的遗传多样性进行分析。【结果】杂交兰240对EST-SSR引物在国兰中的通用性比例分别为春兰(67.92%)、建兰(66.25%)、墨兰(71.25%)。利用其中的17对引物对44份国兰品种资源进行分子系统发育研究,有10对引物表现出多态性,10对引物共扩增出52个条带,多态性条带数为3~9个,多态性信息含量分布范围为0.504~0.850。聚类分析结果表明,44个国兰品种可分为4大聚类群,分类结果与传统的植物学分类相吻合。【结论】本研究发掘的杂交兰EST-SSR引物可以有效用于国兰的分子遗传多样性研究。
【Objective】Cymbidium hybrid EST-SSR markers were studied to determine their transferability for applications on the cultivar identification and germplasm utilization of Chinese cymbidium cultivars.【Method】Genetic diversity of 240 pairs EST-SSR primers in 44 cultivars of Cymbidium goeringii,C.ensifolium,C.sinense and others were analyzed,and their transferability among Chinese cymbidium cultivars examined.【Result】The rates of transferability were found to be 67.92%for C.goeringii,66.25%for C.ensifolium,and 71.25%for C.sinense.Among the markers,17 EST-SSR primer pairs were selected to study the molecular phylogeny of the 44 cultivars.Out of them,10 primer pairs showed polymorphisms with expanded 52 bands.The bands numbered between 3 to 9 with a range of polymorphism information content between 0.504 and0.850.These cultivars were clustered into 4 groups,same as concluded by the conventional botanical classification.【Conclusion】The Cymbidium hybrid EST-SSR markers selected from this study effectively showed the molecular genetic diversity of the various Chinese cymbidium cultivars.
作者
林榕燕
陈艺荃
钟淮钦
吴建设
林兵
LIN Rongyan;CHEN Yiquan;ZHONG Huaiqin;WU Jianshe;LIN Bing(Institute of Crop Sciences,Fujian Academy of Agricultural Sciences/Flower Research Center,Fujian Academy of Agricultural Science/Fujian Engineering Research Center for Characteristic Floriculture,Fuzhou,Fujian 350013,China;Agricultural Engineering and Technology Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2020年第5期503-508,共6页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划公益类专项(2018R1026-3、2017R1026-8)
福建省农业科学院博士基金项目(DC2018-1)
福建省财政专项——福建省农业科学院科技创新团队建设项目(STIT2017-2-9)
福建省农业科学院作物研究所青年开放基金项目(2017QN-1)。
关键词
杂交兰
EST-SSR标记
国兰
通用性
多态性
Cymbidium hybrid
EST-SSR marker
Chinese cymbidium
transferability
polymorphism
