摘要
为探明引进的阿月浑子品种间的亲缘关系,采用RAPD标记技术对引入的12个国外品种和‘新疆优株’进行了分析。用筛选的21条10bp随机引物进行PCR扩增可扩增出137个位点,其中多态位点122个,多态位点比率89.05%;品种间遗传距离在0.2015～0.5163之间,表明各品种间存在一定的遗传差异。UPGMA聚类分析表明,13个阿月浑子品种在遗传距离0.40处可划分为3个类群,第1类包括‘Kerman’、‘Larnaka’、‘M-38’、‘M-P3’、‘Ashoury’、‘N’、‘Joley’、‘Aegina’、‘Avidon’、‘B’和‘Peter’11个品种;第2类为‘Xinjiang selecttree’品种;第3类为‘Mateur’品种。
In order to analyze pistachio genetic relationship,random amplified polymorphic DNA（RAPD） was used to study the classification and identification of 13 pistachio（Pistacia vera L）cultivars which includs 12 exotic varities and one native selects of Xinjiang.Twenty-one 10 bp primers that selected from eighty arbitrary primers were applied for amplifying the pistachio DNA.Total 137 bands were produced,among which 122 bands（89.05%） were polymorphic.Genetic distances among the cultivars were estimated based on the amount of band sharing and ranged from 0.2015-0.5163,it is indicated that there are genetic differences among the cultivars.UPGMA cluster analysis showed that 13 pistachio varieties can be divided into three groups in the genetic distance of 0.40 level：The first category includes eleven varieties,the second category is the ‘Xinjiang selection’ and the third category is ‘Mateur’ varieties.
出处
《园艺学报》
CAS
CSCD
北大核心
2010年第3期475-478,共4页
Acta Horticulturae Sinica
基金
河北省林业局项目(0406260)
国家林业局项目(2006-30)
