摘要
目的探讨淋巴细胞活化基因-3(LAG3)在骨髓增生异常综合征(MDS)患者T细胞亚群中的表达。方法选取2019年1至11月期间于天津医科大学总医院血液科新诊断的MDS患者16例为MDS组,另纳入16名健康成人为健康对照组,采用流式细胞术检测CD8+T细胞、CD4+T细胞和调节性T细胞在MDS组和健康对照组中的表达水平。结果共纳入MDS患者16例;男5例,女11例;中位年龄56(18~80)岁。健康对照组16名,男女各8名,中位年龄40(17~69)岁,两组性别和年龄组成差异无统计学意义(均P>0.05)。LAG3在MDS组CD8+T细胞中的表达显著高于健康对照组(74.45%±22.31%比58.78%±14.82%,P<0.05)。LAG3在MDS组调节性T细胞中的表达显著高于健康对照组(64.91%±10.32%比49.09%±13.58%,P<0.05)。LAG3在CD4+T细胞中的表达MDS组与健康对照组间差异无统计学意义(P>0.05)。结论LAG3在MDS患者CD8+T和调节性T细胞上的表达高于健康人。
Objective To investigate the expression of Lymphocyte activation gene 3(LAG3)in myelodysplastic syndromes(MDS)patients.Methods A total of 16 MDS patients newly diagnosed in Hematology Department of Tianjin Medical University were enrolled from January to November 2019.The healthy control(HC)group includes 16 cases of healthy adults.The expression levels of LAG3 on CD8+T cells,CD4+T cells and regulatory T cells(Treg)in MDS patients and healthy controls were detected by flow cytometry.Results A total of 16 patients with MDS were included,including 5 males and 11 females,with a median age of 56(18-80)years old.HC group includes 16 healthy adults,8 men and 8 women,with a median age of 40(17-69)years.There was no statistically significant difference in gender and age composition between the two groups(both P>0.05).The expression of LAG3 on CD8+T cells in MDS patients(74.45%±22.31%)was significantly higher than that in HC group(58.78%±14.82%,P<0.05).The LAG3 expression on Treg in MDS patients(64.91%±10.32%)were significantly higher than that of HC group(49.09%±13.58%,P<0.05).There was no statistical difference in LAG3 expression on CD4+T cells between the two groups.Conclusion The expression of LAG3 on CD8+T cells and Treg increases in MDS patients than that of healthy people.
作者
谢凝
孙莹莹
江汇涓
刘春燕
邵宗鸿
Xie Ning;Sun Yingying;Jiang Huijuan;Liu Chunyan;Shao Zonghong(Tianjin Medical University,Tianjin 300070,China;Hematology Department,Tianjin Medical University General Hospital,Tianjin 300052,China)
出处
《中华医学杂志》
CAS
CSCD
北大核心
2020年第24期1886-1889,共4页
National Medical Journal of China
基金
国家自然科学基金(81400088、81770118、81870101)
天津市自然科学基金(16JCZDJC35300)。
