摘要
目的了解艾滋病(AIDS)病人合并EB病毒(EBV)及巨细胞病毒(CMV)的感染状况,为临床检测、诊断及治疗提供参考依据。方法收集西南医科大学附属医院感染科收治的263例AIDS病人的血液标本,采用酶联免疫吸附法检测EB病毒衣壳IgM抗体(EBV-CA-IgM)、EB病毒衣壳IgG抗体(EBV-CA-IgG),采用化学发光法检测巨细胞病毒IgM抗体(CMV-IgM)、巨细胞病毒IgG抗体(CMV-IgG),采用荧光定量聚合酶链反应法检测EB病毒脱氧核糖核酸(EBV-DNA)及巨细胞病毒脱氧核糖核酸(CMV-DNA)。统计分析上述指标在不同CD4^+T淋巴细胞(简称CD4细胞)计数的AIDS病人中表达的差异性。结果263例AIDS病人EBV-CAIgG阳性263例(100%),EBV-CA-IgM阳性6例(2.3%),EB-DNA阳性83例(31.6%),CMV-IgG阳性263例(100%),CMV-IgM阳性23例(8.7%),CMV-DNA阳性109例(41.4%)。EBV-CA-IgM及CMV-IgM阳性率在不同组CD4细胞计数病人中的阳性率差异无统计学意义(P>0.05),CD4细胞计数<200个/μL组病人的EBV-DNA阳性率高于CD4细胞计数≥200个/μL组(P<0.05),CD4细胞计数<50个/μL组病人的CMVDNA阳性率明显高于CD4细胞计数≥50个/μL组(P<0.05)。结论AIDS病人合并EBV及CMV感染率较高,在检测特异性抗体的同时应进行病毒DNA的检测以期早发现、早治疗。
Objective To investigate the infection status of EB virus(EBV)and cytomegalovirus(CMV)in patients with AIDS,to provide reference for clinical diagnosis and treatment.Methods Blood samples from 263 AIDS patients admitted to the affiliated hospital of Southwest Medical University were collected.EBV-CA-IgM and EBV-CA-IgG were detected by enzyme-linked immunosorbent assay,detection of CMV-IgM,CMV-IgG by chemiluminescence,and detection of EBVDNA and CMV-DNA by PCR-fluorescence.The differences in expression of the above indicators in patients with different CD4^+T cell counts were statistically analyzed.Results 1.263(100%)AIDS patients were EBV-CA-IgG positive;6(2.3%)were EBV-CA-IgM positive;83(31.6%)were EB-DNA positive;and 263(100%)were CMV-IgG positive.23 cases(8.7%)were CMV-IgM positive,and 109 cases(41.4%)were CMV-DNA positive.2.The positive rates of EBV-CA-IgM and CMVIgM were not significantly different between different groups of CD4^+T cell count(P>0.05).The positive rate of EBVDNA in patients with CD4^+T cell count<200/μL was higher than that in CD4^+T cell count≥200/μL group(P<0.05).The positive rate of CMV-DNA in patients with CD4^+T cell count<50/μL was significantly higher than that in CD4^+T cell count≥50/μL group(P<0.05).Conclusion The rate of AIDS patients with EBV and CMV infection is high,indicating that in the detection of specific antibodies,viral DNA should be detected for early detection and early treatment.
作者
肖科
赵东霞
罗瑜
钟利
XIAO Ke;ZHAO Dongxia;LUO Yu;ZHONG Li(Department of Infectious Diseases,Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan,China)
出处
《中国艾滋病性病》
CAS
CSCD
北大核心
2020年第6期587-589,606,共4页
Chinese Journal of Aids & STD
基金
四川省重点学科(传染病学)建设项目基金(SZD0421)
西南医科大学青年基金(2018-ZRQN-007)。
