摘要
该研究通过紫外线(UV)灭活的黄颡鱼(Pelteobagrus fulvidraco)精子利用冷休克方法诱导兰州鲇(Silurus lanzhouensis)雌核发育二倍体(G2n)、单倍体(N)、杂交二倍体(H2n),以及同源精子诱导的普通二倍体(2N)的胚胎发育时序和发育生物学特征进行了观察比较。结果显示,(1)受精率为2N>H2n>N>G2n,孵化率为2N>G2n>N,畸形率为N>G2n>2N。(2)在水温23°C下,普通二倍体48 h孵化出膜,雌核发育二倍体51 h孵化出膜,杂交二倍体在神经胚期(26 h 20 min)停止发育,单倍体在出膜前期(43 h)停止发育。(3)各组胚胎发育形态上的差别主要表现在杂交二倍体未形成正常胚体,单倍体表现出典型的单倍体综合征,雌核发育二倍体初孵仔鱼在形态学上与普通二倍体无明显差异。该研究为兰州鲇雌核发育技术提供了技术支撑,同时也为单倍体、杂交和雌核发育胚胎的细胞生物学研究提供了理论依据。
In this study,the sperm of Pelteobagrus fulvidraco was UV-irradiated and used to fertilize eggs of Silurus lanzhouensis as well as diploid gynogenesis (G2n) was induced using cold shock.The diploid gynogenesis (G2n),haploid (N),hybrid diploid (H2n),and homodiploid embryo (2N) were compared in development timing and biological characteristics of embryo development.The results were as follows:(1)Fertilization rates were 2N>H2n>N>G2n,Abnormality rates were 2N>G2n>N,Hatching rates were N>G2n>2N.(2)At the temperature of 23 °C,the ordinary diploid embryo hatched out in 48 h,gynogenesis diploid hatched out in 51 h,hybrid diploid ceased to develop in neurula stage (26 h 20 min),and the haploid ceased to develop in pre-hatching stage.(3)The differences in the developmental morphology of the embryos in each group mainly manifested in the fact that the hybrid diploid did not form a normal embryo body,but the haploid showed a typical haploid syndrome.Compared with the ordinary diploid,newly hatched larvae of gynogenetic diploid was no significantly different in morphology.The study provides a method for the gynogenetic technique of S.lanzhouensis,as well as a theoretical basis for cytological studies on the development of haploid,hybridized,and gynogenetic embryos of this species.
作者
李兰兰
肖伟
邢露梅
赛清云
刘彦斌
田永华
王燕
俞兆曦
刘哲
连总强
LI Lanlan;XIAO Wei;XING Lumei;SAI Qingyun;LIU Yanbin;TIAN Yonghua;WANG Yan;YU Zhaoxi;LIU Zhe;LIAN Zongqiang(College of Animal Science and technology,Gansu Agricultural University,Lanzhou 730070,China;Ningxia Fisheries Research Institute,Yinchuan 750001,China;Ningxia Fishery Engineering Technology Research Center,Yinchuan 750001,China;Academician Workstation of Ningxia Fishery Science and Technology,Tinchuan 750001,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2020年第5期820-828,共9页
Chinese Journal of Cell Biology
基金
国家重点科技研发计划“蓝色粮仓”重点专项项目(批准号:2018YFD0901202)
宁夏重点研发计划项目(批准号:2017BN06)资助的课题。
关键词
兰州鲇
雌核发育
单倍体
杂交胚胎
胚胎发育
Silurus lanzhouensis
gynogenesis
haploid
hybrid embryo
embryonic development
