摘要
[目的]以家蚕为试虫,测定昆虫病原线虫(entomopathogenic nematode,EPN)对家蚕的安全性。[方法]EPN对家蚕的侵染试验参照《农药生物活性测试标准操作规范》(杀虫剂卷)所述鳞翅目幼虫喷雾法和浸叶饲喂法进行;EPN对家蚕安全性试验参照《化学农药环境安全评价试验准则》第11部分——家蚕急性毒性试验方法进行。[结果]当浓度为15亿线虫/hm^2时,结果显示存在一定的风险。病原线虫喷施后6 h内取桑叶喂食,可显著影响家蚕虫的结茧率和化蛹率,存在一定的风险,但6 h后的桑叶喂食则对其没有显著影响。[结论]严格控制施药后的间隔时间,EPN制剂可在蚕桑地区使用。
[Aims]This study aims to evaluate the safety of entomopathogenic nematode(EPN)on silkworm by field trial.[Methods]The EPN infestation test on silkworms was conducted in accordance with the spraying method and the leaf-feeding method of Lepidoptera larvae as described in the Standard Practice for Pesticide Biological Activity Testing(Insecticide Volume).the EPN safety test was conducted according to the Guidelines of Chemical Pesticide Environment Safety Evaluation Test Part 11-Silkworm acute toxicity test method.[Results]When the concentration was of 100 million nematode per hectare,the results showed a certain risk that the scarred rate and phlegm rate of silkworm decreased significantly when silkworm was fed with the mulberry leaves within 6 hours after treated with EPN.However,there was no side effect on silkworm fed with treated leaves harvested at interval more than 6 hours.[Conclusions]As a result,the EPN could be used in the area of sericulture as an effective pesticide to control the striped flea beetles under strict interval after application.
作者
杨凤丽
曹婷婷
吴慧明
张斌
YANG Feng-li;CAO Ting-ting;WU Hui-ming;ZHANG Bin(Agricultural Technology Extension Center of Deqing County,Huzhou 313200,Zhejiang,China;Zhejiang Plant Protection and Quarantine Bureau,Hangzhou 310014,China;Zhejiang Agriculture and Forestry University,Hangzhou 311300,China;Green Spirit Natural Enemies Biotechnology Co.,Ltd.,Hangzhou 311200,China)
出处
《农药》
CAS
CSCD
北大核心
2020年第7期502-503,508,共3页
Agrochemicals
基金
浙江省农业农村厅“三农六方”科技协作项目(CTZB-F180706LWZ-SNY1)。
关键词
昆虫病原线虫
家蚕
安全性评价
entomopathogenic nematode(EPN)
silkworm
safety evaluation
