摘要
[目的]本文旨在探究生物质炭表面可溶性组分对作物生长的潜在作用机制。[方法]采用新一代高通量测序手段——RNA-Seq技术对添加生物质炭可溶性组分处理和未添加可溶性组分的不结球白菜叶片进行转录组分析,研究其差异基因的功能,并利用实时荧光定量PCR技术(RT-qPCR)对关键基因进行检测验证。[结果]添加生物质炭可溶性组分的不结球白菜叶片中共检测到499个差异基因,其中上调表达基因152个,下调表达基因347个。RT-qPCR验证分析与转录组测定结果一致。基因功能注释显示差异基因多集中于细胞壁的多糖代谢和碳水化合物代谢等生物过程,主要调控果胶裂解酶、果胶甲酯酶、葡糖基转移酶等活性。[结论]果胶裂解酶和果胶甲酯酶同源基因的下调表达、葡糖基转移酶活性同源基因的上调表达证实生物质炭可溶性组分可以在增加植物细胞壁稳定性的同时提高作物对逆境条件的抵抗能力,从基因表达的角度揭示了生物质炭可溶性组分对不结球白菜的影响。
[Objectives]The aim of this study was to explore the potential biological mechanisms of dissolved components on the surface of biochar for crop growth.[Methods]In this study,a new high-throughput sequencing technology,RNA-Seq,was used to analyze the function of different genes and transcriptome of non-heading Chinese cabbage leaves which were treated with dissolved components of biochar.The key genes were detected and verified by quantitative PCR(RT-qPCR).[Results]499 differentially expressed genes were detected in the leaves of non-heading Chinese cabbage treated with biochar soluble components,of which 152 were up-regulated and 347 were down-regulated.The gene function annotation showed that the differential genes were concentrated in biological processes such as polysaccharide metabolism and carbohydrate metabolism in cell wall,mainly regulating the activities of enzymes such as pectin lyase,pectin methylesterase and glucosyltransferase.[Conclusions]The down-regulated expression of pectin lyase and pectin methylase homologous genes,and the up-regulated expression of glucosyltransferase activity homologous genes confirmed that biochar soluble components could increase plant cell wall stability while improving crop resistance to adversity conditions,revealing the effects of biochar soluble components on non-heading Chinese cabbage growth and metabolism from the perspective of crop gene expression.
作者
时薇
卞荣军
郑聚锋
刘晓雨
张旭辉
李恋卿
潘根兴
SHI Wei;BIAN Rongjun;ZHENG Jufeng;LIU Xiaoyu;ZHANG Xuhui;LI Lianqing;PAN Genxing(Institute of Resource,Ecosystem and Environment of Agriculture,Nanjing Agricultural University,Nanjing 210095,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2020年第4期674-681,共8页
Journal of Nanjing Agricultural University
基金
中央高校基本业务费专项资金(KYZ201713)
国家自然科学基金项目(41877096,41877097)。
关键词
不结球白菜
可溶性组分
基因差异表达
高通量测序
转录组分析
non-heading Chinese cabbage
soluble components
differential gene expression
high-throughput sequencing
transcriptome analysis
