摘要
为研究国内不同繁养基地红螯螯虾群体的遗传结构特征,采用线粒体细胞色素氧化酶亚基Ⅰ(COⅠ)序列直接测序的方法,获取红螯螯虾国内6个繁养群体(安徽阜阳、浙江湖州、广东珠海、江苏镇江、海南澄迈、江苏苏州)共180个样本的COⅠ序列。试验结果显示,序列比对长度为831 bp,其中变异位点56个,简约信息位点16个。基因序列G+C(%)含量较低(39.765%),表现出较明显的碱基组成偏倚性。180个样本共检测到10种单倍型,其中单倍型H1出现次数最多,为安徽阜阳、广东珠海、江苏镇江、海南澄迈、江苏苏州群体的共享单倍型。国内红螯螯虾繁养群体的遗传多样性较高,所有样本的总体单倍型多样性指数为0.693,核苷酸多样性指数为0.00652。其中苏州群体的单倍型多样性指数最高(0.964),海南澄迈群体最低(0.618)。不同群体间的遗传距离为0.00403~0.00969,海南澄迈和安徽阜阳群体间的遗传距离较大(0.00969),广东珠海和江苏苏州群体间遗传距离最小(0.00403)。试验结果表明,国内红螯螯虾繁养群体保持着较高的遗传多样性,群体间存在着一定的基因交流。试验结果为后续合理开发利用国内红螯螯虾种质资源积累了数据资料。
To evaluate the genetic diversity of red claw crayfish Cherax quadricarinatus in different breeding farms in China,180 individuals of six breeding groups(Fuyang in Anhui,Huzhou in Zhejiang,Zhuhai in Guangdong,Zhenjiang in Jiangsu,Chengmai in Hainan,and Suzhou in Jiangsu)were cloned based on mitochondrial cytochrome oxidase subunitⅠgene(COⅠ).Sequence alignment showed that there were 56 variable sites and 16 parsimony-informative sites in total 831 sites.The content of(G+C)was lower(39.765%),showing obvious bias of base composition.Ten haplotypes were detected among the 180 samples.Haplotype H1 found most frequently was shared by the populations from Fuyang,Zhuhai,Zhenjiang,Chengmai and Suzhou.Haplotype diversity index was 0.693 and nucleotide diversity index was 0.00652,indicating that the genetic diversity index of total samples was high.Suzhou population had the highest haplotype diversity index(0.964)while Chengmai population had the lowest haplotype diversity index(0.618)among the six populations.The genetic distance between Chengmai and Fuyang populations was higher than those of others while between Zhuhai and Suzhou is the lowest among the six red claw crayfish groups.The findings indicated that there was high level genetic diversity in red claw crayfish,there were also some gene exchanges among populations.This study has accumulated data for the development and utilization of domestic red claw crayfish germplasm resources.
作者
彭刚
徐宇
张燕
王静
黄鸿兵
严维辉
许志强
PENG Gang;XU Yu;ZHANG Yan;WANG Jing;HUANG Hongbing;YAN Weihui;XU Zhiqiang(Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, China)
出处
《水产科学》
CAS
CSCD
北大核心
2020年第4期615-619,共5页
Fisheries Science
基金
江苏省农业自主创新项目[SCX(18)3381]
江苏省六大人才高峰项目(2015-NY-027)
江苏省水产三新工程项目(Y2017-13)。
