摘要
对沙鳅亚科鱼类3属14个代表种的线粒体DNA控制区序列的结构进行了分析。通过与鲤形目鱼类的控制区序列进行比较,将沙鳅亚科鱼类的控制区分为终止序列区、中央保守区和保守序列区三个区域。同时识别了沙鳅亚科中一系列保守序列,并给出了它们的一般形式。以胭脂鱼为外类群,对比条鳅亚科、花鳅亚科、以及平鳍鳅科的代表性种类,采用NJ、MP和ML法构建沙鳅亚科的分子系统树。分子系统发育分析表明,沙鳅亚科为一单系,包括3个属:沙鳅属、副沙鳅属和薄鳅属,各属均构成单系。根据分子系统学、形态学的结果及地理分布推断,沙鳅亚科中沙鳅属可能为最为原始的属,副沙鳅属其次,而薄鳅属最特化。
Belonging to Cobitidae of Cypriniformes, the subfamily Botiinae is a group of small or middle freshwater fishes dwelling in slow to moderately swirl waters, and many of them are common aquarium species. The classification of Botiinae fishes is controversial among different authors. Fang（1936） and Nalbant（1963） divided Botiinae into two genera, Botia and Leptobotia, while Chen（ 1980）separated it into three, Botia, Parabotia and Leptobotia. Up to now, there was no study conducted on the structure of mitochondrial DNA control region and the molecular phylogenetic relationship of the subfamily Botiinae. In the present study, the structure of the control region sequences was analyzed for fourteen species representing the three genera of Botiinae. Similar to other fishes, the control region of Botiinae is composed of three domains, the extended termination associated sequence （ETAS）domain, the Central conserved domain（ CD） and the Conserved sequence block（CSB）. The ETAS domain is very rich （73.3%） in the contents of A and T, while the CD domain is much rich（36.3%）in G and C. A termination-associated sequence TAS（TACATA）and its reverse complementary sequence（ ATGTAT） were found in the ETAS domain, and the conserved sequence in this domain is ETAS 1. The CD domain is highly conserved, and three conserved sequences were identified, CSB-F, CSB-E and CSB-D. Three conserved sequences were identified in the CSB domain, CSB-1, CSB-2 and CSB-3. With Myxocyprinus asiaticus as outgroup, compared with members of Nemacheilinae, Cobitinae and Homalopteridae as well, the phylogenetic relationship of Botiinae was analyzed using the neighbor-joining（NJ） method in MEGA2.1, the maximum parsimony（MP） method in PAUP^＊ 4.0b10 and the maximum likelihood（ML） method in TREE-PUZZLE 5.0. The results showed that the subfamily Botiinae is monophyletic and the confidence levels in the three analysis methods were 88% in NJ, 57 % in MP and 97% in ML respectively. It also supported that Botiinae c
出处
《水生生物学报》
CAS
CSCD
北大核心
2005年第6期645-653,共9页
Acta Hydrobiologica Sinica
基金
国家自然科学基金(30170137
404320037)
中国科学院创新方向项目(KZCX3-SW-126)资助
