摘要
目的探究芦荟苷对肝癌MHCC97H细胞增殖、迁移及侵袭能力的影响,并探讨其相关分子机制。方法将肝癌MHCC97H细胞随机分为对照组和低、高剂量实验组,实验组分别予以100,200μmol·L^-1芦荟苷处理,对照组予以等量二甲基亚砜(DMSO)处理。通过CCK-8法检测芦荟苷对细胞增殖的影响,细胞划痕及Transwell小室实验模拟观测芦荟苷对细胞迁移和侵袭的影响,蛋白免疫印迹法(Western Blot)检测凋亡相关蛋白表达情况。结果给药24 h后,低、高剂量实验组的细胞增殖抑制率分别为(15.30±0.54)%和(34.25±0.60)%;差异有统计学意义(P<0.05);对照组及低、高剂量实验组的细胞克隆形成率分别为(21.21±0.11)%,(13.73±0.06)%和(8.13±0.04)%;细胞相对迁移率分别为(100.00±2.13)%,(57.84±2.47)%和(24.55±1.52)%;穿膜细胞数目分别为(968.00±45.36)个,(448.70±13.22)个和(37.03±13.05)个,低、高剂量实验组的上述指标与对照组比较,差异均有统计学意义(均P<0.05)。给药48 h后,对照组及低、高剂量实验组B细胞淋巴瘤/白血病-2基因(Bcl-2)蛋白相对表达量分别为1.00±0.05,0.80±0.01和0.47±0.04;B细胞淋巴瘤/白血病-2基因相关X蛋白(Bax)蛋白相对表达量分别为1.00±0.01,1.77±0.01和2.23±0.01;低、高剂量实验组与对照组比较,差异均有统计学意义(均P<0.05)。结论芦荟苷可抑制肝癌MHCC97H细胞增殖、迁移及侵袭能力,促进其凋亡,作用机制与调控Bcl-2/Bax的表达有关。
Objective To investigate the effects of aloin on the proliferation,migration and invasion of hepatocellular carcinoma MHCC97H cells and its related mechanism.Methods Hepatocellular carcinoma MHCC97H cells were randomly divided into control group,test-L group and test-H group.The test-L group and test-H group were treated with 100 and 200μmol·L^-1 of aloin,respectively,while control group was given equal amount of dimethylsulfoxide(DMSO).CCK-8 method was used to analyze the effect of aloin on cell proliferation.Cell scratch and transwell chamber experiments were used to analyze the effect of aloin on cell migration and invasion.The apoptosis associated protein was detected with Western Blot method.Results At 24 h after treatment,the inhibition rates of hepatoma cell proliferation in the test-L group and test-H group were(15.30±0.54)%and(34.25±0.60)%,respectively,with statistically significant difference(P<0.05).The rates of cell clone formation in the control group,test-L group and test-H group were(21.21±0.11)%,(13.73±0.06)%and(8.13±0.04)%,respectively;The relative migration rates of hepatoma cell were(100.00±2.13)%,(57.84±2.47)%and(24.55±1.52)%,respectively;The transmembrane cells were 968.00±45.36,448.70±13.22 and 237.03±13.05,respectively.There were statistically significant differences in the above indices between the test-L/-H group and the control group(P<0.05).At 48 h after treatment,the relative expressions of anti-apoptotic protein b-cell lymphoma-2(Bcl-2)in the control group,test-L group and test-H group were 1.00±0.05,0.80±0.01 and 0.47±0.04,respectively;the relative expression levels of pro-apoptotic protein b-cell lymphoma-2 associated x protein(Bax)were 1.00±0.01,1.77±0.01 and 2.23±0.01,respectively.There were statistically significant differences in the above indices between the test-L/-H group and the control group(P<0.05).Conclusion Aloin can inhibit the proliferation invasion and migration of hepatocellular carcinoma MHCC97H cells,which is related to regulating Bcl-2/Bax expre
作者
杜毅超
郑天翔
石昊
钱保林
陈浩
张浩
谭鹏
夏先明
付文广
DU Yi-chao;ZHENG Tian-xiang;SHI Hao;QIAN Bao-lin;CHEN Hao;ZHANG Hao;TAN Peng;XIA Xian-ming;FU Wen-guang(Academician(Expert)Workstation of Sichuan Province,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,Suchuan Province,China;Department of Hepatobiliary Surgery,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,Suchuan Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2020年第12期1669-1671,共3页
The Chinese Journal of Clinical Pharmacology
基金
四川省科技计划基金资助项目(2018JY0283,2017SZYZF0015)
西南医科大学校级基金资助项目(2018-ZRQN-013)
西南医科大学附属医院科研课题计划基金资助项目(2017-PT-53)。
关键词
芦荟苷
肝癌
增殖
侵袭
迁移
aloin
hepatocellular carcinoma
proliferation
invasion
migration
