摘要
【目的】探讨血清14-3-3η蛋白与抗环瓜氨酸肽(CCP)抗体在类风湿性关节炎(RA)中的诊断价值.【方法】本院收治的RA患者40例作为RA组,同期体检的40例健康体检者作为对照组,40例非RA的关节病变患者设为非RA关节病变组(非RA组).比较三组血清14-3-3η蛋白与抗CCP抗体水平,绘制ROC曲线分析二者对RA的诊断价值.根据病情活动度,将RA患者分成活动期组、缓解期组,比较两组血清14-3-3η蛋白、抗CCP抗体、血沉(ESR)、C反应蛋白(CRP)、类风湿性因子(RF)水平,绘制ROC曲线分析二者对RA病情活动度的预测价值,获得曲线下面积,明确最佳截断值,并获得相应的敏感度、特异度.【结果】RA组血清14-3-3η蛋白、抗CCP抗体、ESR、CRP、RF水平显著高于非RA组、对照组(P<0.05).14-3-3η蛋白、抗CCP抗体、14-3-3η蛋白联合抗CCP抗体诊断RA的曲线下面积分别为0.666、0.712、0.781.在40例RA患者中,活动期25例(62.50%),缓解期15例(37.50%).活动期组14-3-3η蛋白、抗CCP抗体水平显著高于缓解期组(P<0.05).14-3-3η蛋白、抗CCP抗体、14-3-3η蛋白联合抗CCP抗体预测RA病情活动度的曲线下面积分别为0.733、0.745、0.819.【结论】血清14-3-3η蛋白和抗CCP抗体对RA的诊断及病情活动度评估具有一定价值,必要时可将二者联合用于RA诊断,以提高诊断符合率.
【Objective】To explore the diagnostic value of serum 14-3-3ηprotein and anti-cyclic citrullinated peptide(CCP)in rheumatoid arthritis(RA).【Methods】A total of 40 patients with RA admitted to our hospital were selected as RA group,while 40 healthy persons who underwent physical examination in our hospital during the same period were selected as control group.In addition,40 patients with non-RA joint disease were selected as non-RA joint disease group.Serum 14-3-3ηprotein,ES,CR,RF and anti-CCP antibody levels were detected in all three groups,and ROC curve was drawn to analyze their diagnostic value for RA.According to the degree of disease activity,RA patients were divided into active group and remission group.Serum 14-3-3ηprotein and anti-CCP antibody levels were compared between the RA active and remission groups.The ROC curve was drawn to analyze the predictive value on the activity of RA.The area under the curve was obtained,the optimal cutoff value was defined,and the corresponding sensitivity and specificity were calculated.【Results】Serum levels of 14-3-3ηprotein,anti-CCP antibody,ESR,CRP and RF in the RA group were significantly higher than those in the non-RA group and the control group(P<0.05).The areas under the curve of 14-3-3ηprotein,anti-CCP antibody and 14-3-3ηprotein combined with anti-CCP antibody in the diagnosis of RA were 0.666,0.712 and 0.781,respectively.Among 40 patients with RA,25 cases(62.50%)had active phase and 15 cases(37.50%)had remission phase.The levels of 14-3-3ηprotein and anti-CCP antibody in the RA active group were significantly higher than those in RA remission group(P<0.05).The area under the curve of 14-3-3ηprotein,anti-CCP antibody and 14-3-3ηprotein combined with anti-CCP antibody to predict the activity of RA was 0.733,0.745 and 0.819,respectively.【Conclusion】Serum 14-3-3ηprotein and anti-CCP antibody have certain value in the diagnosis of RA and the evaluation of disease activity.If necessary,they can be combined for better diagnosis of RA to improv
作者
王君哲
张小蓉
WANG Jun-zhe;ZHANG Xiao-rong(Clinical Laboratory Chongqitig Three Gorges Central Hospital Chongqing Three Gorges,404000)
出处
《医学临床研究》
CAS
2020年第6期843-846,850,共5页
Journal of Clinical Research
