摘要
采用低共溶剂(deep eutectic solvents,DES)-K2HPO4双水相体系(aqueous two-phase system,ATPS)将香菇多糖水提液中的多糖与蛋白质进行分离萃取,蛋白质富集于DES相,糖类富集于K2HPO4相。综合考察了DES物质的量比、K2HPO4浓度、DES的加入量以及萃取时间对蛋白质脱除率的影响,通过正交试验进一步优化试验条件,得出蛋白质脱除的最优工艺参数:以氯化胆碱/丙三醇(物质的量之比1∶2,2.6 mL)、K2HPO4(0.6 g/mL,3 mL)构建双水相体系,加入2 mL香菇多糖水提液,萃取30 min后,香菇多糖蛋白质的脱除率高达90.8%,香菇多糖回收率为98.0%。在此条件下得到的香菇多糖具有清除DPPH自由基的能力,说明脱蛋白后的香菇多糖具有一定的抗氧化性。
Separation and extraction of polysaccharides and proteins from Lentinus edodes aqueous extract using poly-oligosolvents(DES)-K2HPO4 aqueous two-phase system(ATPS),Protein was enriched in DES phase and carbohydrate was enriched in K2HPO4 phase.The effects of DES molar ratio,K2HPO4 concentration,DES addition and extraction time on protein removal rate were investigated.The orthogonal experiment was used to further optimize the experimental conditions,The optimum technological parameters for protein removal were obtained:The aqueous two-phase system was constructed by choline chloride/glycerol(1∶2,2.6 mL)and K2HPO4(0.6 g/mL,3 mL),When 2 mL lentinan water extract was added,the extraction time was 30 min,the protein removal rate of lentinan was as high as 90.8% and the recovery of lentinan was 98.0%.Lentinan obtained under these conditions had the ability to scavenge DPPH free radicals,indicating that the deproteinized lentinan had certain antioxidant activity.
作者
王莹
邢晓玲
李屿君
王思怡
仲菲
WANG Ying;XING Xiao-ling;LI Yu-jun;WANG Si-yi;ZHONG Fei(Department of Pharmacy,Jiangsu Animal Husbandry and Veterinary Science College,Taizhou 225300,Jiangsu,China)
出处
《食品研究与开发》
CAS
北大核心
2020年第14期98-103,共6页
Food Research and Development
基金
江苏省大学生创新创业训练项目(201912806037Y)。
关键词
低共熔溶剂
香菇多糖
蛋白质
萃取
抗氧化
low eutectic solvents
lentinan
protein
extraction
antioxidant
