摘要
目的建立高效液相色谱法测定板栗种子中γ-氨基丁酸的分析方法。方法板栗样品使用2,4-二硝基氟苯乙腈进行衍生化前处理后,以磷酸缓冲溶液(pH=7)、乙腈和水为流动相,采取梯度洗脱的方式进行测定,柱温35℃,流速1.0 mL/min,进样量20μL,使用紫外检测器,测定波长为360 nm。结果本方法标准曲线呈现良好的线性关系,相关系数达到0.9996。方法的回收率为109%,相对标准偏差为0.4%,γ-氨基丁酸最小检出限0.05μg/mL。精密度实验得出5次进样的相对标准偏差为0.55%。对3种不同品种的板栗种子进行测定时,3种样品的RSD分别为3.077%、3.219%、5.333%,平行性较好。结论该方法快速、易操作,具有较高的测量精密度和准确度,对实际样品进行测试时能避免杂质峰的干扰,重现性好,能够满足对板栗种子中γ-氨基丁酸含量的检测要求。
Objective To establish a method for the determination of teraminobutyric acid in chestnut seeds by high performance liquid chromatography.Methods After the sample was derivatized by 2,4-dinitrofluorobenzene,it was determined by gradient elution with phosphate buffer solution(pH=7),acetonitrile and water as mobile phases.The column temperature was selected at 35℃,the flow rate was 1.0 mL/min,the injection volume was 20μL,and the detection wavelength was 360 nm with ultraviolet detector.Results The standard curve of this method showed a good linear relationship with a correlation coefficient of 0.9996.The recoveries were 109%,the relative standard deviations was 0.4%,and the limit of quantitation was 0.05μg/mL.The precision experiment showed that the relative standard deviation of 5 injections was 0.55%.When the seeds of 3 different varieties of chestnut were measured,the RSD of the three samples were 3.077%,3.219%,and 5.333%,respectively,with good parallelism.Conclusion The proposed method is fast and easy to operate,with high precision and accuracy of measurement,which can avoid the interference of impurity peaks when testing actual samples.It has good reproducibility,and is suitable for detecting γ-amino butyric acid in chestnut seeds.
作者
高茜
杜常健
史胜青
江泽平
韩振泰
GAO Qian;DU Chang-Jian;SHI Sheng-Qing;JIANG Ze-Ping;HAN Zhen-Tai(Research Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091,China;State Forestry Administration Key Laboratory of Forest Ecology and Environment,Beijing 100091,China;Research Institute of Forestry Chinese Academy of Forestry,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration,Beijing 100091,China)
出处
《食品安全质量检测学报》
CAS
2020年第11期3593-3597,共5页
Journal of Food Safety and Quality
基金
中央级公益性科研院所基本科研业务费专项(CAFRIFEEP201407)
国家林业和草原局科技发展中心项目(KJZXSA2019045)
中国林科院林业所林木培育重点实验室专项(ZDRIF201712)。
关键词
高效液相色谱法
Γ-氨基丁酸
板栗种子
high performance liquid chromatography
γ-amino butyric acid
chestnut seeds
