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不同产地牛大力水提物中总黄酮、总皂苷及总多糖的含量测定 被引量:10

Content Determination of Total Flavonoids,Saponins and Polysaccharides in Millettia Speciosa Champ.from Different Producing Areas
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摘要 目的:比较不同产地牛大力中总黄酮、总皂苷及总多糖的含量差异,为其质量评价及应用提供参考依据。方法:选取广东、广西、海南、福建产地的牛大力,以水为溶剂,采用加热回流提取,通过UV法测定其水提物中总黄酮、总皂苷及总多糖含量。结果:总黄酮线性范围为32.70~87.20μg·ml^-1(r=0.9994),平均回收率为98.87%,RSD为1.44%(n=6),样品含量:海南万宁>广东云浮>福建古田>广西钦州。总皂苷线性范围为15.83~158.33μg·ml^-1(r=0.9974),平均回收率为98.56%,RSD为1.93%(n=6),样品含量:海南万宁>广西钦州>广东云浮>福建古田。总多糖线性范围为1.98~9.91μg·ml^-1(r=0.9995),平均回收率为98.88%,RSD为1.92%(n=6),样品含量:海南万宁>广东云浮>广西钦州>福建古田。结论:不同产地牛大力的成分含量不一,以海南的品质较佳;可作为食补药材用于养生保健。 Objective:To compare the contents of total flavonoids,total saponins and total polysaccharides in Millettia speciosa Champ.from different producing areas to provide reference for its quality evaluation and application.Methods:Millettia speciosa Champ.from Guangdong,Guangxi,Hainan and Fujian provinces were extracted by heating reflux with water as the solvent.The contents of total flavonoids,saponins and polysaccharides were determined by ultraviolet spectrophotometry.Results:The linear range of total flavonoids was 32.70-87.20μg·ml^-1(r=0.9994),and the average recovery was 98.87%and the RSD was 1.44%(n=6).The order of the content in the samples was as follows:Hainan Wanning>Guangdong Yunfu>Fujian Gutian>Guangxi Qinzhou.The linear range of total saponins was 15.83-158.33μg·ml^-1(r=0.9974),and the average recovery was 98.56%and the RSD was 1.93%(n=6).The order of the content in the samples was as follows:Hainan Wanning>Guangxi Qinzhou>Guangdong Yunfu>Fujian Gutian.The linear range of total polysaccharides was 1.98-9.91μg·ml^-1(r=0.9995),and the average recovery was 98.88%and the RSD was 1.92%(n=6).The order of the content in the samples was as follows:Hainan Wanning>Guangdong Yunfu>Guangxi Qinzhou>Fujian Gutian.Conclusion:The component contents of Millettia speciosa Champ.from different producing areas are different,and the samples from Hainan exhibit better quality,and can be used as food tonic for health care.
作者 李荣宇 林少玲 骆月姬 苏靖雯 Li Rongyu;Lin Shaolin;Luo Yueji;Su Jingwen(The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Health Management Center of China Southern Airlines)
出处 《中国药师》 CAS 2020年第6期1208-1210,共3页 China Pharmacist
关键词 牛大力 产地 含量 总黄酮 总皂苷 总多糖 紫外分光光度法 Millettia speciosa Champ. Producing areas Content Total flavonoids Total saponins Total polysaccharides UV spectrophotometry
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