摘要
目的探讨hsa-miR-150-5p在其基因家族的进化关系及可能介入的生物学过程。方法利用人类miRNA表达数据库(HMED)获得hsa-miR-150-5p在不同组织和疾病中特异性表达丰度信息,运用miRBase、Clustalw2和MEGA5.0分析软件获取hsa-miR-150-5p的染色体定位、碱基序列比对特征和进化规律等基本信息,通过miRDB、PicTar和TargetScan进行靶基因预测,采用基因本体(GO)和代谢通路富集分析(KEGG)对靶基因的生物学功能注释分析,进一步认识hsa-miR-150-5p的生物学功能。结果通过同源性搜索在miRBase数据中获得miR-150基因家族成员的序列36条,分布于23个物种。多序列比对发现miR-150基因家族成员序列碱基保守性很高,其中有16个碱基完全保守,6个碱基相对保守。进化分析表明人类的miR-150与青鳉、三文鱼、钳鱼、斑马鱼的分子系统进化关系较远,与其他18个物种的进化关系较近。对miRDB、PicTar和TargetScan预测的靶基因进行交集后共得到25个靶基因,GO分析结果显示靶基因参与多个信号通路的调节,包括转录因子、锌指蛋白、Toll样受体信号通路的转导蛋白、ATP偶联的离子型通道受体和细胞因子信号传导抑制蛋白等。结论 hsa-miR-150-5p在多种组织和疾病中表达,分布具有组织特异性,可能在机体的多种生理、病理过程中发挥重要作用,并可能通过调控多个靶基因而参与各个信号通路调节。
Objective To investigate the evolutionary relationship of has-miR-150-5p in the gene family and biological processes it may be involved.Methods The expression abundance of hsa-miR-150-5p in different tissues and diseases was obtained from HMED.The chromosomal location,sequence characteristic and molecular evolution of hsa-miR-150-5p were analyzed by miRBase,Clustalw2 and MEGA5.0.MiRDB,PicTar and TargetScan were used to predict target genes of hsa-miR-150-5p.The gene ontology(GO)and KEGG were used to analyze the hsa-miR-150-5p function annotation.Results By sequence similarity searching,36 sequences of members of the miR-150 gene family were obtained from miRBase,which were distributed in 23 species.Multiple sequence alignment revealed that the sequence of the members of the miR-150 gene family was highly conserved,16 of which were completely conserved and 6 were highly conserved.Phylogenetic analysis showed that human miR-150 had far evolutionary relationship with Oryzias latipes,Salmo salar,Ictalurus punctatus and Danio rerio,and close relationship with other 18 species.Twenty-five target genes of hsa-miR-150-5p were predicted from the intersection of miRDB,PicTar and TargetScan.GO analysis showed that the target genes participated in regulation of multiple signal paths,including Transcription factors,zinc finger proteins,Toll-like receptor signaling pathway transduction proteins,ATP-coupled ionic channel receptors and inhibitors of cytokine signal transduction.Conclusion Hsa-miR-150-5p is expressed in multiple tissues and diseases,which has tissue-specific gene expression.It has suggested that hsa-miR-150-5p may play an important role in physiological and pathological processes of the body and involved in the regulation of various signaling pathways by regulating multiple target genes.
作者
李九龙
刘林
张湘
蔡雪梅
何德超
刘宇欣
钟晓武
龙璠
LI Jiulong;LIU Lin;ZHANG Xiang;CAI Xuemei;HE Dechao;LIU Yuxin;ZHONG Xiaowu;LONG Fan(Department of Clinical Laboratory,the Fifth People′s Hospital of Nanchong,Nanchong,Sichuan 637100,China;Department of Clinical Laboratory,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637000,China)
出处
《检验医学与临床》
CAS
2020年第13期1805-1810,共6页
Laboratory Medicine and Clinic
基金
四川省科技厅应用基础项目(2017JY0151)
四川省南充市市校科技战略合作项目(18SXHZ0513,NSMC20170436)
四川省卫生健康委员会科研课题(19PJ200)。
